The inhomogeneous refractive indices of tissues blur and distort single molecule emission patterns generating image artifacts and decreasing the achievable resolution of single molecule localization microscopy (SMLM). We developed deep learning driven adaptive optics (DL-AO) that monitors the individual emission patterns from SMLM experiments, infers their shared wavefront distortion, and drives a deformable mirror to compensate sample induced aberrations. We demonstrated that DL-AO compensates 28 types of wavefront deformation shapes, restores single molecule emission patterns approaching the conditions untouched by the specimen, and improves the resolution and fidelity of 3D SMLM through brain tissues over 130 µm, with 3-20 mirror changes.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.