This contribution will present a variety of applications of lab-on-a-chip surface enhanced Raman spectroscopy in the
field of bioanalytic. Beside the quantification and online monitoring of drugs and pharmaceuticals, determination of
enzyme activity and discrimination of bacteria are successfully carried out utilizing LOC-SERS. The online-monitoring
of drugs using SERS in a microfluidic device is demonstrated for nicotine. The enzyme activity of thiopurine
methyltransferase (TPMT) in lysed red blood cells is determined by SERS in a lab-on-a-chip device. To analyse the
activity of TPMT the metabolism of 6-mercaptopurine to 6-methylmercaptopurine is investigated. The discrimination of
bacteria on strain level is carried out with different E. coli strains. For the investigations, the bacteria are busted by ultra
sonic to achieve a high information output. This sample preparation provides the possibility to detect SERS spectra
containing information of the bacterial cell walls as well as of the cytoplasm. This contribution demonstrates the great
potential of LOC-SERS in the field of bioanalytics.
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