We give an overview of the design of a metrological scanning probe microscope (mSPM) currently under development at the National Measurement Institute Australia (NMIA) and report on preliminary results on the implementation of key components. The mSPM is being developed as part of the nanometrology program at NMIA and will provide the link in the traceability chain between dimensional measurements made at the nanometer scale and the realization of the International System of Units (SI) meter at NMIA. The instrument is based on a quartz tuning fork (QTF) detector and will provide a measurement volume of 100 μm×100 μm×25 μm with a target uncertainty of 1 nm for the position measurement. Characterization results of the nanopositioning stage and the QTF detector are presented along with an outline of the method for tip mounting on the QTFs. Initial imaging results are also presented.
We give an overview of the design of a metrological Scanning Probe Microscope (mSPM) currently under development
at the National Measurement Institute Australia (NMIA) and report on preliminary results on the implementation of key
components. The mSPM is being developed as part of the nanometrology program at NMIA and will provide the link in
the traceability chain between dimensional measurements made at the nanometre scale and the realization of the SI metre
at NMIA. The instrument is based on a quartz tuning fork (QTF) detector and will provide a measurement volume of
100 μm × 100 μm × 25 μm with a target uncertainty of 1 nm for the position measurement. Characterization results of the
nanopositioning stage and the QTF detector are presented along with an outline of the method for tip mounting on the
QTFs. Initial imaging results are also presented.
We have explored controlled movement of magnetic beads and a dumbbell structure composed of DNA, a magnetic and a non-magnetic bead in a micro fluidic channel. Movement of the beads and dumbbells is simulated assuming that a net force is described as a superposition between the magnetic and hydrodynamic drag forces. Trajectories of beads and dumbbells are observed with optical light microscopy. The experimentally measured data show a good agreement with the simulations. This dynamical approach offers the prospect to stretch the DNA within the dumbbell and investigate its conformational changes. Further on, we demonstrate that short sonication can reduce multiple attachments of DNA to the beads.
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