Mr. Bo Liu Profile

Bo Liu

at Purdue Univ

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Publications (9)

Proceedings Article | 26 February 2010 Paper

Molecular imaging of neutropilin-1 receptor using photoacoustic spectroscopy in breast tumors

Keith Stantz, Minsong Cao, Bo Liu, Kathy Miller, Lili Guo

Proceedings Volume 7564, 75641O (2010) https://doi.org/10.1117/12.842271

KEYWORDS: Tumors, Photoacoustic spectroscopy, Near infrared, In vivo imaging, Receptors, Luminescence, Calibration, Breast, Absorption, Molecular imaging

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Proceedings Article | 24 February 2010 Paper

Ex vivo hemoglobin status study using photoacoustic computed tomography small animal scanner

Bo Liu, Robert Kruger, Daniel Reinecke, Keith Stantz

Proceedings Volume 7564, 75643A (2010) https://doi.org/10.1117/12.842836

KEYWORDS: Blood, Oxygen, Scanners, Photon transport, Error analysis, Absorption, Monte Carlo methods, Molecules, Acoustics, Imaging systems

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Proceedings Article | 24 February 2010 Paper

Monitor hemoglobin concentration and oxygen saturation in living mouse tail using photoacoustic CT scanner

Bo Liu, Robert Kruger, Daniel Reinecke, Keith Stantz

Proceedings Volume 7564, 756439 (2010) https://doi.org/10.1117/12.842827

KEYWORDS: Oxygen, Scanners, Spectroscopy, Arteries, Photoacoustic spectroscopy, Blood, Acoustics, Veins, Bone, Imaging spectroscopy

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Proceedings Article | 24 February 2010 Paper

Effects of radiation on tumor hemodynamics and NF-kappaB in breast tumors

Keith Stantz, Ning Cao, Bo Liu, Minsong Cao, Helen Chin-Sinex, Marc Mendonca, Jian Jian Li

Proceedings Volume 7564, 75641J (2010) https://doi.org/10.1117/12.842238

KEYWORDS: Tumors, Oxygen, Photoacoustic spectroscopy, Hemodynamics, Radiation effects, In vivo imaging, Imaging spectroscopy, Breast, Breast cancer, Computed tomography

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Proceedings Article | 13 February 2007 Paper

Phantom and in-vivo measurements of hemoglobin concentration and oxygen saturation using PCT-S small animal scanner

Bo Liu, Daniel Reinecke, Robert Kruger, Keith Stantz

Proceedings Volume 6437, 64371X (2007) https://doi.org/10.1117/12.701376

KEYWORDS: Blood, Oxygen, Scanners, Absorption, Tumors, In vivo imaging, Veins, Arteries, Hypoxia, Calibration

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Proceedings Article | 13 February 2007 Paper

Using Monte Carlo simulations to understand the influence of photon propagation on photoacoustic spectroscopic imaging

Keith Stantz, Bo Liu, Robert Kruger

Proceedings Volume 6437, 64370Z (2007) https://doi.org/10.1117/12.701395

KEYWORDS: Monte Carlo methods, Tumors, Blood, Photoacoustic spectroscopy, Absorption, Near infrared, Tissue optics, Photon transport, Acoustics, Signal attenuation

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Purpose: The purpose of this study is to evaluate the influence of photon propagation on the NIR spectral features associated with photoacoustic imaging. Introduction: Photoacoustic CT spectroscopy (PCT-S) has the potential to identify molecular properties of tumors while overcoming the limited depth resolution associated with optical imaging modalities (e.g., OCT and DOT). Photoacoustics is based on the fact that biological tissue generates high-frequency acoustic signals due to volume of expansion when irradiated by pulsed light. The amplitude of the acoustic signal is proportional to the optical absorption properties of tissue, which varies with wavelength depending on the molecular makeup of the tissue. To obtain quantifiable information necessitate modeling and correcting for photon and acoustic propagation in tumors. Material and Methods: A Monte Carlo (MC) algorithm based on MCML (Monte Carlo for Multi-Layered edia) has been developed to simulate photon propagation within objects comprised of a series of complex 3D surfaces (Mcml3D). This code has been used to simulate and correct for the optical attenuation of photons in blood, and for subcutaneous tumors with homogenous and radially heterogeneous vascular distributions. Results: The NIR spectra for oxygenated and deoxygenated blood as determined from Monte Carlo simulated photoacoustic data matched measured data, and improving oxygen saturation calculations. Subcutaneous tumors with a homogeneous and radially heterogeneous distribution of blood revealed large variations in photon absorption as a function of the scanner projection angle. For select voxels near the periphery of the tumor, this angular profile between the two different tumors appeared similar. Conclusions: A Monte Carlo code has been successfully developed and used to correct for photon propagation effects in blood phantoms and restoring the integrity of the NIR spectra associated with oxygenated and deoxygenated blood. This code can be used to simulate the influence of intra-tumor heterogeneity on the molecular identification via NIR spectroscopy.

Proceedings Article | 20 March 2006 Paper

Evaluating dynamic contrast-enhanced and photoacoustic CT to assess intra-tumor heterogeneity in xenograft mouse models

Keith Stantz, Bo Liu, Minsong Cao, Dan Reinecke, Mario Dzemidzic, Yun Liang, Robert Kruger

Proceedings Volume 6143, 61431F (2006) https://doi.org/10.1117/12.654056

KEYWORDS: Tumors, Oxygen, Blood, Photoacoustic spectroscopy, Breast, In vivo imaging, Vascular physiology, Calibration, Error analysis, Plasma

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Proceedings Article | 6 March 2006 Paper

Assessment of photoacoustic computed tomography to classify tissue in a polycystic-kidney disease mouse model

Bo Liu, Vincent Gattone, Robert Kruger, Keith Stantz

Proceedings Volume 6086, 608607 (2006) https://doi.org/10.1117/12.646180

KEYWORDS: Kidney, Tissues, Mouse models, Tissue optics, Image segmentation, Photons, Photoacoustic tomography, Photography, Computed tomography, Absorption

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Proceedings Article | 6 March 2006 Paper

Photoacoustic spectroscopic imaging of intra-tumor heterogeneity and molecular identification

Keith Stantz, Bo Liu, Minsong Cao, Daniel Reinecke, Kathy Miller, Robert Kruger

Proceedings Volume 6086, 608605 (2006) https://doi.org/10.1117/12.645106

KEYWORDS: Tumors, Blood, Photoacoustic spectroscopy, In vivo imaging, Spectroscopy, Error analysis, Oxygen, Liver, Tissue optics, Bladder

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Purpose. To evaluate photoacoustic spectroscopy as a potential imaging modality capable of measuring intra-tumor heterogeneity and spectral features associated with hemoglobin and the molecular probe indocyanine green (ICG). Material and Methods. Immune deficient mice were injected with wildtype and VEGF enhanced MCF-7 breast cancer cells or SKOV3x ovarian cancer cells, which were allowed to grow to a size of 6-12 mm in diameter. Two mice were imaged alive and after euthanasia for (oxy/deoxy)-hemoglobin content. A 0.4 mL volume of 1 μg/mL concentration of ICG was injected into the tail veins of two mice prior to imaging using the photoacoustic computed tomography (PCT) spectrometer (Optosonics, Inc., Indianapolis, IN 46202) scanner. Mouse images were acquired for wavelengths spanning 700-920 nm, after which the major organs were excised, and similarly imaged. A histological study was performed by sectioning the organ and optically imaging the fluorescence distribution. Results. Calibration of PCT-spectroscopy with different samples of oxygenated blood reproduced a hemoglobin dissociation curve consistent with empirical formula with an average error of 5.6%. In vivo PCT determination of SaO₂ levels within the tumor vascular was measurably tracked, and spatially correlated to the periphery of the tumor. Statistical and systematic errors associated with hypoxia were estimated to be 10 and 13%, respectively. Measured ICG concentrations determined by contrast-differential PCT images in excised organs (tumor, liver) were approximately 0.8 μg/mL, consistent with fluorescent histological results. Also, the difference in the ratio of ICG concentration in the gall bladder-to-vasculature between the mice was consistent with excretion times between the two mice. Conclusion. PCT spectroscopic imaging has shown to be a noninvasive modality capable of imaging intra-tumor heterogeneity of (oxy/deoxy)-hemoglobin and ICG in vivo, with an estimated error in SaO₂ at 17% and in ICG at 0.8 μg/mL in excised tissue. Ongoing development of spectroscopic analysis techniques, probe development, and calibration techniques are being developed to improve sensitivity to both exogenous molecular probes and (oxy/deoxy)-hemoglobin fraction.

Showing 5 of 9 publications

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