This article developed a calibration method to evaluate the metrological characteristics of non-amplified nucleic acid quantitative fluorometers, by studying the working principle of non-amplified nucleic acid quantitative fluorometer. Multiple different types of non-amplified nucleic acid quantitative fluorometers were selected for pre-testing and national certified reference materials are chosen as reference. Critical parameters such as indication error, repeatability, and correlation coefficient are selected as test items. The experimental results shows that, the method proposed and reference materials selected can effectively evaluate the metrological performance of the instrument, which is of great significance for the quality control and quantity traceability of non-amplified nucleic acid quantitative fluorometers.
This article presents a novel design of reference light source and physical calibration method for optical performance evaluation of micro-plate chemiluminescence analyzers in order to substitute for traditional biological and biochemical methods. The reference light source is designed according to the working principle, and technical characteristics such as repeatability, stability, interference among wells, differences among channels and linearity of micro-plate chemiluminescence analyzers. The calibration results of 4 typical types of micro-plate chemiluminescence analyzers show that the reference light source and physical calibration method presented in this article can well simulate different chemiluminescence reagents and meet the requirements of luminous intensity calibration for chemiluminescence analyzers with the advantages of simple structure, good applicability, low cost and accurate and reliable measurement results.
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