SignificanceDiverse behaviors rely on coordinated activity and multi-regional functional connectivity within astrocyte-neuronal networks. However, current techniques for simultaneously measuring astrocytic and neuronal activities across multiple brain regions during behaviors remain limited.AimWe propose a multi-fiber solution that can simultaneously record activities of astrocyte-neuronal networks across multiple regions during behaviors.ApproachWe employed cell-specific dual-color genetically encoded calcium indicators (GECIs) and multi-fiber photometry to simultaneously measure astrocytic and neuronal Ca2+ transients across multiple brain regions in freely behaving animals.ResultsOur findings demonstrate that both movements and sensory stimuli induce synchronized and highly correlated Ca2+ transients in astrocytes and neurons of freely behaving mice. In addition, we recorded astrocytic and neuronal Ca2+ transients from multiple brain regions during mouse behaviors. Our observations reveal heightened synchronization of astrocytic and neuronal Ca2+ transients across different brain regions during movements or sensory stimuli, indicating enhanced functional connectivity within brain-wide astrocyte-neuronal networks.ConclusionsMulti-fiber photometry, combined with cell-specific dual-color GECIs, represents a powerful approach for investigating astrocytic and neuronal activities across different brain regions during behaviors. This technique serves as a versatile tool for analyzing the multi-regional functional connectivity map of astrocyte-neuronal networks associated with specific behaviors.
Blast-induced TBI (bTBI) is a type of traumatic brain injury generated by a shock wave that causes the head to quickly accelerate or decelerate. It has huge challenges in the diagnosis and prognosis due to its absence of significant edema and traumatic areas. The major diagnosis methods for bTBI has some defects such as time-consuming, poor specificity, low sensitivity. Thus, it is highly desirable to establish a rapid and high-sensitivity method for the detection of bTBI. Raman spectroscopy technique has been used in biochemistry due to its label-free and non-destructive. It is expected to detect and monitor the progression and regression of bTBI from molecular perspective. In this paper, the Raman spectra of the hippocampus and hypothalamus tissue were measured at 3h, 6h, 24h, 48h and 72h after mild and moderate bTBI in 2800-3000cm-1 range. The results showed both the two brain areas had a significant difference in intensity of Raman characteristic peaks at 2855, 2885 and 2934cm-1 at different time points compared with the sham group. It demonstrates that the content of the lipids and proteins have been changed in the rat hippocampus and hypothalamus after bTBI, due to the Raman peaks at 2855, 2885 and 2934cm-1 assigning to C-H stretching of lipids and protein. It infers that Raman spectroscopy technique has the potential to be a rapid and effective diagnosis and monitoring method in bTBI clinic.
In this paper, a novel strategy for diagnosis of bTBI is proposed by measuring terahertz (THz) spectroscopy of serum in a rat model. A compressed air-driven shock wave generator was used to establish bTBI model of rats. It was validated by a neurological deficit assessment method. The spectral differences of serum for different degrees of bTBI rats have been demonstrated by THz time-domain spectroscopy (THz-TDS). Moreover, based on the THz spectra of serum, the principal component analysis (PCA) and support vector machine (SVM) were performed to automatically identify the degrees of bTBI. The results indicate that THz spectroscopy could provide a sensitive, rapid and label-free diagnosis method for bTBI.
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