KEYWORDS: Cornea, Collagen, Second-harmonic generation, Microscopy, Transmission electron microscopy, Tissues, Signal detection, Pathology, Harmonic generation, RGB color model
We implement polarization-resolved second harmonic generation microscopy to characterize the orientation distribution of collagen lamellae in human cornea. We evidence a less ordered distribution in keratoconic corneas, in agreement with their deteriorated mechanical behavior.
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