3D reconstruction of the human brain at high resolution is one of the most important challenges of neuroscience. We present a new clearing method named SHORT that in combination with an advanced double-view light-sheet fluorescence microscope and an automated machine-learning based images analysis allow to perform volumetric study of the human brain. We applied our methodology to a Broca’s area block of 4 x 4 x 2 cm3, demonstrating the possibility of obtaining a fast 3D reconstruction of the human brain at high-resolution, paving the way to the possibility of finally mapping a comprehensive atlas of the human brain.
This conference presentation, “3D molecular phenotyping of the human brain Broca’s area using light-sheet fluorescence microscopy” was prepared for the Biomedical Spectroscopy, Microscopy, and Imaging II conference at SPIE Photonics Europe 2022.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.