We report an objective-lens-free endomicroscopic catheter for compact Lissajous scanned confocal endomicroscopic system using a scanning lensed-fiber. The fiber scanner comprises a single-mode fiber (SMF) spliced with a coreless silica fiber segment and quadrupole piezoelectric tube. An objective lens was directly formed at the distal end of an SMF using a fusion splicer. The lensed fiber eliminates the need for conventional beam focusing elements such as a gradient index rod lens and precise optical alignment, allowing low cost and facile fabrication of an exceptionally short endomicroscopic catheter. The endomicroscopic scanner was fully packaged within a stainless tube of 2.6 mm in outer diameter and 20 mm in length, which can be easily inserted through the working channel of conventional laparoscope or colonoscope. The microscopic images of a stained kidney section and ear of a mouse were successfully obtained with the Lissajous scanning confocal endomicroscope. The compact Lissajous scanning lensed fiber can provide a new route for diverse in vivo endomicroscopic applications.
KEYWORDS: Imaging systems, Confocal microscopy, Scanners, In vivo imaging, Endomicroscopy, Microscopes, Indocyanine green, Luminescence, Field programmable gate arrays, Active optics
This work presents a fully packaged confocal endomicroscopic system using Lissajous fiber scanner for in-vivo imaging. The confocal endomicroscopic system consists of a scanning probe part, an optical part, and an electrical part. The scanning probe uses resonant Lissajous fiber scanner based on a piezoelectric tube. The scanner successfully achieves 10 frame rate with ~ 1 kHz scanning frequencies. The probe was fully packaged for waterproofing and disinfection of medical instruments into the outer diameter of 3.4 mm. The endomicroscopic system and successfully obtained 2D reflectance imaging results, human ex-vivo imaging results and a real-time in-vivo imaging results.
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