KEYWORDS: Optical tweezers, Calibration, Green fluorescent protein, Sensors, In vivo imaging, Refraction, In vitro testing, Optical calibration, Luminescence, Modulation
Retinal pigment epithelial (RPE) cells play an integral role in the renewal of photoreceptor disk membranes. As rod and
cone cells shed their outer segments, they are phagocytosed and degraded by the RPE, and a failure in this process can
result in retinal degeneration. We have studied the role of myosin VI in nonspecific phagocytosis in a human RPE
primary cell line (ARPE-19), testing the hypothesis that this motor generates the forces required to traffic phagosomes in
these cells. Experiments were conducted in the presence of forces through the use of in vivo optical trapping. Our
results support a role for myosin VI in phagosome trafficking and demonstrate that applied forces modulate rates of
phagosome trafficking.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.