KEYWORDS: Stereoscopy, 3D image processing, Nanoparticles, Luminescence, Gold, Scattering, Light scattering, Ultrasonography, Signal to noise ratio, Signal generators
We will present the initiative in imaging a hugely expanded drosophila brain via expansion microscopy, which has brought unprecedented opportunities and challenges. 100X expansion has been demonstrated in enlarging the typically 0.6x0.3x0.2 mm brain to 60x30x20 mm, which promises electron microscopy resolution (approximately 3 nm) with optical microscopy (approximately 300 nm) to resolve the synapses connection. A highly sped-up imaging method integrated with an ultrasound-activated microtome is crucial to support the initiative. Critically, photon statistics set the fundamental considerations in selecting the contrasts for optical imaging.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.