Plasmonic resonance energy transfer (PRET) based imaging has been proposed as an effective label-free real-time biomolecular absorption spectroscopic method that enables the detection of biomolecules and dynamic cell activities. Here, we present a metasurface-based PRET-imaging platform that effectively engineers the darkfield scattering spectrum using the gap plasmon and grating effect. As a demonstration, three different biomolecules (Cyt c, Chl a, Chl b) are simultaneously detected using our metasurface-based PRET-imaging platform. Our proposed metasurface-based PRET-imaging platform enables continuous monitoring in the spatial region and multiplexed PRET sensing of multiple biomolecules with different absorption spectrums in the visible.
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