Gold nanoparticles have been shown to possess x-ray attenuation higher than that of clinically used iodine based contrast
agents. Additionally, gold nanoparticles have also been used in enhancing the Raman signal through a phenomenon
called Surface Enhanced Raman Scattering (SERS). The development of Gold nanoparticle based probes (nanotags) for
combined SERS and x-ray computed tomography (CT) are reported. These nanotags comprise quasi-spherical gold
nanoparticles encoded using a Raman active molecule and encapsulated by a monolayer of polyethylene glycol with
carboxylate functional groups for bioconjugation. The nanotags, made from ~65 nm gold nanoparticles, display large
SERS cross-section and x-ray absorption which are used for dual modality imaging of tumor cells in of an orthotropic
prostate cancer tumor animal model.
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