We describe the molecular engineering of new highly efficient two-photon photolabile cages, based on dissymmetrical
donor-acceptor and symmetrical donor-donor systems. Complete characterizations of these new cages, based on a
biphenyl, fluorenyl or a 2,5-diphenylthienyl central core will be presented. In vitro photochemistry will be described
(uncaging quantum yield, two-photon uncaging action cross-section,...) and we will then focus on biological results
obtained in cell culture and on acute brain slices (with glutamate and fluorescence uncaging).
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