Maite Insausti, Javier Salado, Idoia Castellanos, Luis Lezama, Izaskun Gil de Muro, Jesús M. de la Fuente, Eneko Garayo, Fernando Plazaola, Teófilo Rojo
Magnetite based nanoparticles functionalized with different ligands have been obtained by optimization of two synthetic
methods. Gold surrounded Fe3O4 nanoparticles capped with oleic acid and oleylamine were achieved by thermal
decomposition of metallo-organic precursors. By this way nanoparticles with perfectly defined size within 3.5 nm to 7
nm in diameter and organic content from 16.1% to 40.9 % were obtained. Precipitation of iron(II) chloride in basic
solution yield magnetite nanoparticles between 20 and 40 nm with contents of organic ligands of 3 - 12 %. The samples
have been characterized by X-ray diffraction, transmission electron microscopy and thermogravimetric measurements. A
complete magnetic study has been performed by means of a SQUID magnetometer and electron magnetic resonance
(EMR), showing the influence of capping covering on the superparamagnetic behaviour. The citotoxicity and interaction
with HeLa cells was evaluated for some of the preparations. Finally, the specific absorption rate (SAR) was calculated to
compare the efficiency of heating each sample for the various applied magnetic fields.
During the last decade, there has been great deal of interest in the self-assembly fabrication of hybrid materials from
inorganic nanoparticles and biomolecules. Nanoparticles are similar in size range to many common biomolecules, thus,
nanoparticles appear to be natural companions in hybrid systems. At present, it is straightforward to control and modify
properties of nanostructures to better suit their integration with biological systems; for example, controlling their size,
modifying their surface layer for enhanced aqueous solubility, biocompatibility, or biorecognition. A particularly
desirable target for therapeutic uses is the cell nucleus, because the genetic information is there. We review in this article
the synthesis developed by our research group of water-soluble gold nanoparticles and CdS nanocrystals functionalized
with a Tat protein-derived peptide sequence by straightforward and economical methodologies. The particles were
subsequently tested in vitro with a human fibroblast cell line using optical and transmission electron microscopy to
determine the biocompatibility of these nanoparticles and whether the functionalization with the cell penetrating peptide
allowed particles to transfer across the cell membrane and locate into the nucleus.
Conference Committee Involvement (8)
Colloidal Nanoparticles for Biomedical Applications XII
28 January 2017 | San Francisco, California, United States
Colloidal Nanoparticles for Biomedical Applications XI
13 February 2016 | San Francisco, California, United States
Colloidal Nanoparticles for Biomedical Applications X
7 February 2015 | San Francisco, California, United States
Colloidal Nanoparticles for Biomedical Applications IX
1 February 2014 | San Francisco, California, United States
Colloidal Nanoparticles for Biomedical Applications VIII
2 February 2013 | San Francisco, California, United States
Colloidal Nanocrystals for Biomedical Applications VII
21 January 2012 | San Francisco, California, United States
Colloidal Quantum Dots/Nanocrystals for Biomedical Applications VI
22 January 2011 | San Francisco, California, United States
Colloidal Quantum Dots for Biomedical Applications V
23 January 2010 | San Francisco, California, United States
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