Mr. Jiahao Ma Profile

Jiahao Ma

at Harbin Institute of Technology

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Proceedings Article | 6 December 2022 Paper

Bioinformatic analysis for structure and function of Glutamine synthetase (GS) from Antarctic sea ice bacterium Pseudoalteromonas

Jiahao Ma, Guotong Xu, Le Ao, Siqi Chen, Jingze Liu

Proceedings Volume 12458, 124580O (2022) https://doi.org/10.1117/12.2661100

KEYWORDS: Proteins, Biological research, Bioinformatics, Bacteria, Nitrogen, Chemical analysis, Mode conditioning cables, Microorganisms, Analytical research, Oceanography

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Background: Glutamine synthetases are considered to be an important enzyme class for microbial adaptation to extreme environments, and studying the Antarctic sea ice bacterial glutamine synthetases can be used to explore the molecular mechanisms of psychrophilic microorganisms. Objective: This work use bioinformatics tools to predict structure and function of Glutamine synthetase (GS) from Pseudoalteromonas sp., and to provide a theoretical basis for further study. Methods: Open reading frame (ORF) of GS sequence from Pseudoalteromonas sp. was obtained by ORF finder and was translated into amino acid residue. The structure domain was analyzed by Blast. By the method of analysis tools: Protparam, ProtScale, SignalP-4.0, TMHMM, SOPMA, SWISS-MODEL, NCBI SMART-BLAST and MAGA 7.0, the structure and function of the protein were predicted and analyzed. Results: The results showed that the sequence was GS with 468 amino acid residues, theoretical molecular weight was 51986.64 Da. The protein has the closest evolutionary status with Shewanella oneidensis. Then it had no signal peptide site and transmembrane domain. Secondary structure of GS contained 35.04% α-helix, 16.67% Extended chain, 5.34% β-turn, 42.95% RandomCoil. Conclusions: This GS was a variety of biological functions of protein that may be used as a molecular samples of microbial nitrogen metabolism in extreme environments.

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