A novel polymeric nanoparticle was developed between the negatively charged dendrimer phthalocyanine and positively triblock copolymer for the use as an effective photosensitizer in photodynamic therapy. The intracellular uptake of dendrimer phthalocyanines in HeLa cell was significantly enhanced by encapsulated into nanoparticles. The photocytotoxicity of dendrimer phthalocyanines incorporated into polymeric micelles was also increased. The presence of nanoparticles located induced efficient cell death.
Phthalocyanine complexes are promising second-generation photosensitizers. It has many advantages in photodynamic therapy: good photothermal stability, absorption wavelength in the near-infrared region, and low cytotoxicity. However, they also have some disadvantages:easy to aggregate, poor in solubility, and lack tissue-target.Therefore, how to modify the structure of phthalocyanine is becoming a research hotspot. In this paper, a novel polyfluoroalkylyate silicon (IV) phthalocyanine (SiPc-F) was synthesized. The photophysical properties of this novel polyfluoroalkylyate silicon (IV) phthalocyanine complex (SiPc-F) were studied by UV-visible spectroscopy, steady state and time-resolved spectroscopic methods. The effect of the fluorine substituent on the photophysical properties of phthalocyanine complexes and the quantum yield of singlet oxygen was compared. The results show that the maximum absorption wavelength of polyfluoroalkylyate silicon (IV) phthalocyanine is red-shifted and the intensity is enhanced, the fluorescence intensity becomes stronger, fluorescence quantum yields and fluorescence lifetime is higher but singlet oxygen quantum yields was lower than that of non-fluorinated silicon (IV) phthalocyanine at the same concentration. Polyfluoroalkylyate silicon (IV) phthalocyanine complexes are excellent candidate photosensitizers for photodynamic therapy.
The phosphatase and tensin homolog on chromosome 10 (PTEN) is one of important tumor suppressor proteins in ovarian cancer via negatively regulating the phosphatidylinositol 3-kinase–AKT signaling pathway and controlling genomic stability. Recent studies showed the physiological function of PTEN was closely related with its subcellular compartments. But only a few technologies could quantitatively measure the concentration of PTEN at different subcellular compartments in living cells. In this study, we used fluorescence correlation spectroscopy to measure the concentrations and dynamics of EGFP-PTEN in ovarian cancer cells HO-8910. Our results showed the increasing concentration of PTEN in the cytoplasm had an opposite trends with the nucleus after the oxidative stress stimulation which was induced by H2O2. Furthermore, the altered diffusion of PTEN at different subcellular compartments also illustrated the PTEN was trafficked from the cytoplasm to nucleus.
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