We proposed the imaging-type two-dimensional Fourier spectroscopy that is the phase-shift interferometry
between the objective lights. The proposed method can measure the two-dimensional spectral image on the
focal plane. We construct the proposed method by the infrared radiation optical system to try to apply for the
noninvasive measurement of the blood glucose level. In this report, we discuss about the spectroscopy method
to measure the vessel area of the proximal skin surface that is little affected by the diffusion cased by the
biological membrane.
We proposed the imaging type 2-D Fourier spectroscopy that has the phase shifter on the Fourier
transform plane. The objective lights from the transmitted component and the scattered component of
the measurement object are dispersed on the different area of the Fourier transform plane. Thus, by
introducing the spatial filter, we can emphatically observe the spectral characteristics of the scattered
component light. And the spectral characteristics of the transmitted component and the scattered
component are separately analyzed. But to apply this proposed spectroscopy to biological membrane,
the transmitted light is also diffused by complex refractive index distribution of membrane. Hence, to
control the directionality of the diffused reflected light, we propose the modified illumination method.
By this proposed method, since the diffused reflected light form the diffracted light, we can control
the diffused light as diffracted light to apply for the biological membrane.
We study on the imaging technology of three-dimensional distribution for sugar chain on single living cell-membrane. This technology can observe the entire cell surface. To observe the cell surface, the local area image of cell-membrane is taken by TIRF (total internal reflection fluorescence) microscopy. And by scanning the whole cell surface area, we can obtain the image of the entire cell membrane. These observed local area images can be converted into an entire surface image by the pattern matching processing. For this scanning technology, we propose the proximal two beam optical tweezers to rotate the single floating cell. This proximal two beam optical tweezers can rotate the floating single cell in the nutrient medium by light pressure. Two beams illuminate the single cell at proximal two points from below and above. The cell is trapped at the center of these two focal points. At the same time, light pressures that are generated at two focal points are made to act as rotational torque. Conventionally TIRF microscope is well known as the observation technology for the cell-membrane using the evanescent light as the exciting light. We can observe the local area images of the fluorescently labeled sugar chain that binds the glycoprotein. Using the proposed optical system, we can obtain the fluorescent distribution images on the cell-membrane.
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