Linear Structured Illumination is a powerful technique for increasing the resolution of a fluorescence microscope by a
factor of two beyond the diffraction limit. Previously this technique has only been used to image fixed samples because
the implementation, using a mechanically rotated fused silica grating, was too slow. Here we describe a microscope
design, using a ferroelectric spatial light modulator to structure the illumination light, capable of linear structured
illumination at frame rates up to 11Hz. We show live imaging of GFP labeled Tubulin and Kinesin in Drosophila S2
cells.
KEYWORDS: Picture Archiving and Communication System, Digital imaging, Cancer, Molybdenum, Data storage, Image retrieval, Data communications, Software development, Human-machine interfaces, Prototyping
Although PACS has been studied in industrial countries for more than sixteen years, few effort has been made so far to apply PACS technology to such developing areas as most rural regions in CHina. Engineering and clinical problems raised from installing PACS in undeveloped areas has brought many challenges to our system design. In this study, we try to analyze the clinical need of the Chinese hospitals and find out a solution to meet most hospitals' requirements from engineering and clinical points of view, such as system cost, ease of deployment, user interface, maintenance difficulty, etc., thus provide patients with better medical serve at affordable cost. First of all, we analyzed data collected from Zhejiang Cancer Hospital (ZCH) and take hospitals' financial situation into consideration. then according to the analysis, we made our choices for hardware and software platform, digitizer and storage device. The development of mini PACS is interacted by clinicians and radiologists from ZCH. They showed great interest in the downsizing PACS that does not risk much of the system performance. It is promising that our solution will be accepted in developing areas.
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