Steady-state and time-resolved photoluminescence measurements are powerful tools for getting in-depth information about the nature, characteristics, and environment of proteins and small biomolecules. The spectral region between 280 – 300 nm is significant for biology, life and materials science. Here we present the differences in steady state and time-resolved fluorescence measurements when using a regular pulsed UV-LED and new pulsed high-power UV-LED with a photoluminescence spectrometer.
Investigations of photovoltaic devices and semiconductors are essential to enhance the efficiency of preparation methods as well as their electronic and optical properties. We present a powerful combination of time-resolved photoluminescence microscopy with a spectrometer, which results in a powerful toolbox for researcher. This combination of microscopic (e.g., FLIM, PLIM or carrier diffusion imaging) and spectroscopic methods like wavelength dependent emission scanning enables investigations of photophysical properties of semiconductors, nanoparticles and nanostructures on a whole new level.
Photophysical detection, identification and characterization of nanoparticles, quantum dots and single emitter
are essential to enhance the efficiency of preparation methods as well as their electronic and optical properties. We present a powerful combination of time-resolved photoluminescence microscopy with a spectrometer, which results in a valuable toolbox for researcher. This combination of microscopic (e.g., FLIM, PLIM or antibunching) and spectroscopic methods like wavelength dependent emission scanning enables a deeper understanding for the optimization of properties and efficiencies in practical applications.
Up-conversion nanoparticles are highly attractive for application cases in bio sensing and imaging without autofluorescence. Characterizing the photophyiscial properties of such nanoparticles is essential to enhance the efficiency of preparation methods as well as their electronic and optical properties. We will demonstrate the performance of a spectrometer-microscope assembly for characterization and analysis of up-conversion nanoparticles in terms of lifetime, spectral, and spatial resolution, which provides more information than when using only lifetime or steady-state experiments.
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