Dr. Lisa M Reece Profile

Dr. Lisa M Reece

at Univ of Texas Medical Branch

SPIE Involvement:

Author

Publications (25)

Proceedings Article | 5 March 2015 Paper

A microfabricated microfluidic bioMEMS device to model human brain aneurisms: the aneurysm-on-a-chip

Lisa Reece, Jian Wei Khor, Raviraj Thakur, Ahmed Amin, Steven Wereley, James Leary

Proceedings Volume 9320, 93200P (2015) https://doi.org/10.1117/12.2076037

KEYWORDS: Particles, Microfluidics, BioMEMS, Data modeling, Blood vessels, Instrument modeling, Blood, Hemodynamics, Microfluidic imaging, 3D modeling

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Proceedings Article | 13 March 2013 Paper

Human organ-on-a-chip BioMEMS devices for testing new diagnostic and therapeutic strategies

James Leary, Jaehong Key, Pierre-Alexandre Vidi, Christy Cooper, Ayeeshik Kole, Lisa Reece, Sophie Lelièvre

Proceedings Volume 8615, 86150A (2013) https://doi.org/10.1117/12.2004447

KEYWORDS: Tumors, Magnetic resonance imaging, Diagnostics and therapeutics, Tissues, 3D modeling, Nanoparticles, Breast cancer, Molecules, Tumor growth modeling, Diagnostic tests

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Proceedings Article | 15 February 2011 Paper

Microfluidic MEMS hand-held flow cytometer

Meggie M. G. Grafton, Teimour Maleki, Michael Zordan, Lisa Reece, Ron Byrnes, Alan Jones, Paul Todd, James Leary

Proceedings Volume 7929, 79290C (2011) https://doi.org/10.1117/12.874299

KEYWORDS: Blood, Microfluidics, Magnetism, Scanning probe microscopy, Light emitting diodes, Particles, Point-of-care devices, Data acquisition, Ultraviolet light emitting diodes, Optical design

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Proceedings Article | 25 February 2010 Paper

High-throughput magnetic flow sorting of human cells selected on the basis of magnetophoretic mobility

Lisa Reece, Lehanna Sanders, David Kennedy, Byron Guernsey, Paul Todd, James Leary

Proceedings Volume 7568, 75680P (2010) https://doi.org/10.1117/12.842956

KEYWORDS: Magnetism, Particles, Blood, Stem cells, Patents, Flow cytometry, Luminescence, Transplantation, Statistical analysis, Magnetic tracking

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We have shown the potential of a new method for optimizing the separation of human stem cell subsets from peripheral blood based on a novel cell labeling technique that leverages the capabilities of a new commercially available high speed magnetic cell sorting system (IKOTECH LLC, New Albany, IN). This new system sorts cells in a continuously flowing manner using a Quadrupole Magnetic cell Sorter (QMS). The sorting mechanism is based upon the magnetophoretic mobility of the cells, a property related to the relative binding distributions of magnetic particles per cell, as determined by the utilization of a Magnetic Cell Tracking Velocimeter (MCTV). KG-1 cells were competitively labeled with anti-CD34 magnetic beads and anti-CD34 FITC to obtain an optimal level of magnetophoretic mobility as visualized by the MCTV for high throughput sort recovery in the QMS. In QMS sorting, the concept of split-flow thin channel (SPLITT) separation technology is applied by having a sample stream enter a vertical annular flow channel near the channel's interior wall followed by another sheath flow entering near the exterior wall. The two flows are initially separated by a flow splitter. They pass through the bore of a Halbach permanent quadrupole magnet assembly, which draws magnetized cells outward and deflects them into a positive outflow, while negative cells continue straight out via the inner flow lamina. QMS sorts cells based upon their magnetophoretic mobility, or the velocity of a cell per unit ponderomotive force, the counterpart of fluorescence intensity in flow cytometry. The magnetophoretic mobility distribution of a cell population, measured by automated MCTV, is used as input data for the algorithmic control of sample, sheath, and outlet flow velocities of the QMS. In this study, the relative binding distributions of magnetic particles per cell were determined by MCTV using novel sorting and sizing algorithms. The resulting mobility histograms were used to set the QMS flow parameters so that desired cell populations could be selected on the basis of a mobility "window". The MCTV and the QMS are able to work together to provide good sort boundaries for cell populations that are mathematically defined as opposed to the traditional magnetic sort systems that solely rely on whether a cell is simply "magnetized" or not. One long-term application of this new high speed cell sorting system is to sterilely isolate large numbers of human stem cells directly from a donor's blood for subsequent manipulation in tissue culture for regenerative medicine within that same patient. This will eliminate the need for immune suppressive drugs in an autologous transplantation procedure.

Proceedings Article | 18 February 2010 Paper

Portable microfluidic cytometer for whole blood cell analysis

Meggie Grafton, Michael Zordan, Han-Sheng Chuang, Pooja Rajdev, Lisa Reece, Pedro Irazoqui, Steven Wereley, Ron Byrnes, Paul Todd, James Leary

Proceedings Volume 7593, 75930M (2010) https://doi.org/10.1117/12.842932

KEYWORDS: Blood, Microfluidics, Magnetism, Particles, Avalanche photodetectors, Light emitting diodes, Lab on a chip, Optical filters, Microscopes, Prototyping

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Proceedings Article | 24 February 2009 Paper

A microfluidic-based hybrid SPR/molecular imaging biosensor for the multiplexed detection of foodborne pathogens

Michael Zordan, Meggie Grafton, Ghanashyam Acharya, Lisa Reece, Arthur Aronson, Kinam Park, James Leary

Proceedings Volume 7167, 716706 (2009) https://doi.org/10.1117/12.808316

KEYWORDS: Pathogens, Microfluidics, Imaging systems, Luminescence, Prototyping, Biosensors, Surface plasmons, Imaging devices, Prisms, Gold

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Proceedings Article | 24 February 2009 Paper

Microfabrication of a two-stage BioMEMS microfluidic cell sorter

Meggie Grafton, Benjamin Geheb, Jae Hyuk Jang, Han-Sheng Chuang, Pooja Rajdev, Lisa Reece, Pedro Irazoqui, Steven Wereley, Byunghoo Jung, James Leary

Proceedings Volume 7207, 72070A (2009) https://doi.org/10.1117/12.809854

KEYWORDS: Microfluidics, Digital signal processing, Avalanche photodetectors, Light emitting diodes, Particles, Data acquisition, Microscopes, Microfabrication, BioMEMS, Optical filters

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Proceedings Article | 13 March 2008 Paper

Peptide targeting of quantum dots to human breast cancer cells

Emily Haglund, Mary-Margaret Seale-Goldsmith, Deepika Dhawan, Jane Stewart, Jose Ramos-Vara, Christy Cooper, Lisa Reece, Timothy Husk, Donald Bergstrom, Deborah Knapp, James Leary

Proceedings Volume 6866, 68660S (2008) https://doi.org/10.1117/12.764107

KEYWORDS: Nanoparticles, Tumors, Quantum dots, Tissues, Cancer, Breast cancer, Nanomedicine, In vivo imaging, Therapeutics, In vitro testing

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Proceedings Article | 5 March 2008 Paper

An efficient method to produce clonal colonies of cancer cells using laser enabled analysis and processing (LEAP)

Michael Zordan, Ray Fatig, Lisa Reece, V. Jo Davisson, James Leary

Proceedings Volume 6859, 685910 (2008) https://doi.org/10.1117/12.764069

KEYWORDS: Cancer, Laser ablation, Laser processing, Luminescence, Tumors, Flow cytometry, Real time imaging, Image analysis, Pulsed laser operation, In vitro testing

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Proceedings Article | 29 February 2008 Paper

Design of a multi-stage microfluidics system for high-speed flow cytometry and closed system cell sorting for cytomics

Meggie Grafton, Lisa Reece, Pedro Irazoqui, Byunghoo Jung, Huw Summers, Rashid Bashir, James Leary

Proceedings Volume 6859, 68590V (2008) https://doi.org/10.1117/12.764037

KEYWORDS: Microfluidics, Avalanche photodetectors, Digital signal processing, Computer aided design, Signal processing, Semiconducting wafers, Electronics, Light emitting diodes, Flow cytometry, Luminescence

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Proceedings Article | 19 February 2007 Paper

Comparison of multidimensional flow cytometric data by a novel data mining technique

James Leary, Jacob Smith, Peter Szaniszlo, Lisa Reece

Proceedings Volume 6441, 64410N (2007) https://doi.org/10.1117/12.700940

KEYWORDS: Data mining, Fluorescence correlation spectroscopy, Luminescence, Data analysis, Flow cytometry, Visualization, Human-machine interfaces, Statistical analysis, Pattern recognition, Cancer

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Proceedings Article | 10 February 2007 Paper

Multifunctional nanoparticles for drug/gene delivery in nanomedicine

Mary-Margaret Seale, Dimitry Zemlyanov, Christy Cooper, Emily Haglund, Tarl Prow, Lisa Reece, James Leary

Proceedings Volume 6447, 64470E (2007) https://doi.org/10.1117/12.701645

KEYWORDS: Nanoparticles, Iron, Oxides, Molecules, Multilayers, Magnetism, Manufacturing, Nanomedicine, Cancer, Particles

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Proceedings Article | 6 February 2007 Paper

Design of programmable multilayered nanoparticles with in situ manufacture of therapeutic genes for nanomedicine

Mary-Margaret Seale, Emily Haglund, Christy Cooper, Lisa Reece, James Leary

Proceedings Volume 6430, 643003 (2007) https://doi.org/10.1117/12.701619

KEYWORDS: Nanoparticles, Nanomedicine, Magnetism, Manufacturing, Therapeutics, Particles, In vitro testing, Cell death, Multilayers, Nanotechnology

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Proceedings Article | 1 April 2005 Paper

High-throughput flow cytometric screening of combinatorial chemistry bead libraries for proteomics and drug discovery

James Leary, Lisa Reece, Xian-Bin Yang, David Gorenstein

Proceedings Volume 5692, (2005) https://doi.org/10.1117/12.589456

KEYWORDS: Proteins, Flow cytometry, Chemistry, Drug discovery, Luminescence, Mass spectrometry, Polymers, Microscopy, Molecules, Manufacturing

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Proceedings Article | 1 April 2005 Paper

Biosensor-controlled gene therapy/drug delivery with nanoparticles for nanomedicine

Tarl Prow, William Rose, Nan Wang, Lisa Reece, Yuri Lvov, James Leary

Proceedings Volume 5692, (2005) https://doi.org/10.1117/12.589422

KEYWORDS: Nanoparticles, Molecules, Nanomedicine, Biosensors, Multilayers, Neptunium, Particles, Cancer, Manufacturing, Cell death

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Proceedings Article | 29 March 2005 Paper

Subtractive clustering analysis: a novel data mining method for finding cell subpopulations

Jacob Smith, Lisa Reece, Peter Szaniszlo, Rosemary Leary, James Leary

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.589463

KEYWORDS: Data mining, Luminescence, Analytical research, Biological research, Data modeling, Fluorescence correlation spectroscopy, Human-machine interfaces, Cell death, Flow cytometry, Image processing

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Proceedings Article | 1 July 2004 Paper

Nanomedicine: nanoparticles, molecular biosensors, and targeted gene/drug delivery for combined single-cell diagnostics and therapeutics

Tarl Prow, Jose Salazar, William Rose, Jacob Smith, Lisa Reece, Andrea Fontenot, Nan Wang, R. Stephen Lloyd, James Leary

Proceedings Volume 5318, (2004) https://doi.org/10.1117/12.547922

KEYWORDS: Nanoparticles, Molecules, Nanomedicine, Biosensors, Cell death, Diagnostics and therapeutics, Medicine, Diagnostics, In vitro testing, Nanocrystals

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Proceedings Article | 4 June 2002 Paper

Importance of high-throughput cell separation technologies for genomics/proteomics-based clinical diagnostics

James Leary, Peter Szaniszlo, Tarl Prow, Lisa Reece, Nan Wang, David Asmuth

Proceedings Volume 4625, (2002) https://doi.org/10.1117/12.469773

KEYWORDS: Blood, Magnetism, Statistical analysis, Diagnostics, Proteins, Tissues, Biomedical optics, Modulation, Flow cytometry, Logic

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Gene expression microarray analyses of mixtures of cells approximate a weighted average of the gene expression profiles (GEPs) of each cell type according to its relative abundance in the overall cell sample being analyzed. If the targeted subpopulation of cells is in the minority, or the expected perturbations are marginal, then such changes will be masked by the GEP of the normal/unaffected cells. We show that the GEP of a minor cell subpopulation is often lost when that cell subpopulation is of a frequency less than 30 percent. The GEP is almost always masked by the other cell subpopulations when that frequency drops to 10 percent or less. Several methodologies can be employed to enrich the target cells submitted for microarray analyses. These include magnetic sorting and laser capture microdissection. However, high-throughput flow cytometry/cell sorting overcomes many restrictions of experimental enrichment conditions. This technology can also be used to sort smaller numbers of cells of specific cell subpopulations and subsequently amplify their mRNAs before microarray analyses. When purification techniques are applied to unfixed samples, the potential for changes in gene levels during the process of collection is an additional concern. High-throughput cell separation technologies are needed that can process the necessary number of cells expeditiously in order to avoid such uncontrolled changes in the target cells GEP. In cases where even the use of HTS yields only a small number of cells, the mRNAs (after reverse transcription to cDNA's) must be amplified to yield enough material for conventional microarray analyses. However, the problem of using microamplification PCR methods to expand the amount of cDNAs (from mRNAs) is that it is very difficult to amplify equally all of the mRNAs. Unequal amplification leads to a distorted gene expression profile on the microarray. Linear amplifications is difficult to achieve. Unfortunately, present-day gene-chips need to be about 100 times more sensitive than they are now to be able to do many biologically and biomedically meaningful experiments and clinical tests.

Proceedings Article | 28 May 2002 Paper

Advanced real-time classification methods for flow cytometry data analysis and cell sorting

James Leary, Lisa Reece, James Hokanson, Judah Rosenblatt

Proceedings Volume 4622, (2002) https://doi.org/10.1117/12.468345

KEYWORDS: Digital signal processing, Statistical analysis, Signal processing, Data analysis, Neural networks, Tumors, Flow cytometry, Bone, Principal component analysis, Stem cells

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Proceedings Article | 22 May 2001 Paper

High-throughput cell analysis and sorting technologies for clinical diagnostics and therapeutics

James Leary, Lisa Reece, Peter Szaniszlo, Tarl Prow, Nan Wang

Proceedings Volume 4255, (2001) https://doi.org/10.1117/12.426754

KEYWORDS: Tumors, Blood, Diagnostics, Pathogens, Luminescence, Diagnostics and therapeutics, Flow cytometry, Energy transfer, Breast cancer, Fetus

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Proceedings Article | 10 May 2001 Paper

Real-time decision making for high-throughput screening applications

James Leary, James Hokanson, Judah Rosenblatt, Lisa Reece

Proceedings Volume 4260, (2001) https://doi.org/10.1117/12.426774

KEYWORDS: Data storage, Logic, Classification systems, Complex systems

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Proceedings Article | 27 April 2000 Paper

Application of a new novel data-mining technique to cytometry data

James Leary, Scott McLaughlin, Lisa Reece

Proceedings Volume 3921, (2000) https://doi.org/10.1117/12.384236

KEYWORDS: Data mining, Cell death, Data analysis, Data processing, Flow cytometry, Fluorescence correlation spectroscopy, Mining, Principal component analysis, Algorithm development, Internal medicine

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Proceedings Article | 11 April 2000 Paper

Application of advanced cytometric and molecular technologies to minimal residual disease monitoring

James Leary, Feng He, Lisa Reece

Proceedings Volume 3913, (2000) https://doi.org/10.1117/12.382038

KEYWORDS: Tumors, Statistical analysis, Flow cytometry, Signal processing, Bone, Monoclonal antibodies, Classification systems, Breast cancer, Receivers, Systems modeling

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Proceedings Article | 1 June 1999 Paper

Real-time multivariate statistical classification of cells for flow cytometry and cell sorting: a data mining application for stem cell isolation and tumor purging

James Leary, Scott McLaughlin, Lisa Reece, Judah Rosenblatt, James Hokanson

Proceedings Volume 3604, (1999) https://doi.org/10.1117/12.349196

KEYWORDS: Tumors, Statistical analysis, Data mining, Stem cells, Visualization, Flow cytometry, Data analysis, Bone, Error analysis, Biological research

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Proceedings Article | 21 April 1999 Paper

Detection and isolation of single tumor cells containing mutated DNA sequences

James Leary, Feng He, Lisa Reece

Proceedings Volume 3603, (1999) https://doi.org/10.1117/12.346730

KEYWORDS: Tumors, Breast cancer, Flow cytometry, Blood, Bone, Cancer, Statistical analysis, Transplantation, Systems modeling, Stem cells

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Showing 5 of 25 publications

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