The study reports an investigation of the photoproducts obtained by exposure of chlorpromazine hydrochloride in ultrapure water (concentration 2 mg/mL) to a 266-nm laser beam obtained by fourth harmonic generation from a Nd:YAG laser (6-ns full time width at half maximum, 10-Hz pulse repetition rate). The photoproducts were analyzed by steady-state UV-Vis absorption, laser-induced fluorescence, Fourier transform infrared spectroscopy, and liquid chromatography–tandem time-of-flight mass spectroscopy. Two figures showing pathways that take place during irradiation for obtaining the final products are shown. The quantum yield of singlet oxygen generation by chlorpromazine (CPZ) was determined relative to standard Zn-phthalocyanine in dimethyl sulfoxide. To outline the role of fluorescence in photoproducts formation rates, fluorescence quantum yield of CPZ during exposure to 355-nm radiation (third harmonic of the fundamental beam of Nd:YAG laser) was investigated relative to standard Coumarin 1 in ethanol. The CPZ solutions exposed 60 and 240 min to 266-nm laser beam, respectively, were tested against Staphylococcus aureus ATCC 25923 strain. For 25 μL of CPZ samples irradiated 240 min, a higher diameter of inhibition has obtained against the tested strain than for the 60-min exposed ones.
Phenothiazine exposed to white light or UV radiation undergoes a variety of reactions that result in the degradation of the parental compound and the formation of new species. Chlorpromazine exposed to the 266 nm laser beam of given energy levels yielded species derived from it, whose number increased with the exposure duration. At distinct time intervals the irradiation products were evaluated by spectrophotometry between 200-1500 nm, Thin Layer Chromatography, and for antimicrobial activity of Chlorpromazine against different test organisms such as Staphylococcus aureus.
One of the alternatives to the existing medicines and treatment procedures in fighting multi drug
resistance (MDR) is strengthening the effects of medicines by modifying their molecular structures through
exposure to laser radiation. A method associated with this, is the generation of micro-droplets which contain
medicines solutions; the droplets are utilized/produced as vectors to transport the medicines to targets.
In our studies we try to combine these two methods in order to obtain a new technique to deliver the
efficient medicines to targets that can be applied for a relative large number of chemicals. For this purpose we
have developed an experimental set-up containing a liquid droplets generator, a tunable laser source used to
irradiate droplets, a subunit to measure the laser induced fluorescence (LIF) signals and a real time recording
system for droplet image analysis.
Measurements on different probes, like ultrapure water, commercial grade medicines, newly
developed medicines and laser dyes were performed.. All these measurements were performed on waterbased
solutions.
We present in this paper the laser induced fluorescence measurements results on medicine solutions
(in bulk or in a micro-droplet form) that exhibit important modifications after the exposure at laser radiation. It
was evidenced that the exposures to laser beams/coherent optical radiation of some medicines solutions in
ultrapure water may produce molecular modifications in solutions. These slight modifications of the molecules
made them more efficient against bacteria strains.
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