This work describes online programmable microfluidic bioprocessing units using digital logic microelectrodes for rapid pipelined translocation of DNA molecules and other charged biopolymers as well as nanoparticles. Fundamentals of the design and fabrication technique both the silicon-PDMS and a polyimide-PDMS based construction (a new method based on conventional printed circuit board materials) of these electronic microfluidic devices and their functions are described as well as the experimental results along with the first proof of principle functionality. The electronically controlled collection, separation and channel transfer of the biomolecules and nanosized beads are monitored by a sen-sitive fluorescence setup and controlled by a custom-designed hardware for camera-control and feature selection. This hybrid reconfigurable architecture couples electronic and biomolecular information processing via a single module combination of fluidics and electronics and opens new fields of applications not only in DNA computing and molecular diagnostics but also in applications of combinatorial chemistry and lab-on-a-chip biotechnology to the drug discovery process.
This work focuses on the development of an online programmable microfluidic bioprocessing unit (BioModule) using digital logic microelectrodes for rapid pipelined selection and transfer of DNA molecules and other charged biopolymers. The design and construction technique for this hybrid programmable biopolymer processing device is presented along with the first proof of principle functionality. The electronically controlled collection, separation and channel transfer of the biomolecules is monitored by a sensitive fluorescence setup. This hybrid reconfigurable architecture couples electronic and biomolecular information processing via a single module combination of fluidics and electronics and opens new fields of applications not only in DNA computing and molecular diagnostics but also in applications of combinatorial chemistry and lab-on-a-chip biotechnology to the drug discovery process. Fundamentals of the design and silicon-PDMS-based construction of these electronic microfluidic devices and their functions are described as well as the experimental results.
The goal of this research is to improve the modular stability and programmability of DNA-based computers and in a second step towards optical programmable DNA computing. The main focus here is on hydrodynamic stability. Clockable microreactors can be connected in various ways to solve combinatorial optimisation problems, such as Maximum Clique or 3-SAT. This work demonstrates by construction how one micro-reactor design can be programmed to solve any instance of Maximum Clique up to its given maximum size (N). It reports on an implementation of the architecture proposed previously. This contrasts with conventional DNA computing where the individual sequence of biochemical operations depends on the specific problem. In this pilot study we are tackling a graph for the Maximum Clique problem with N<EQ12, with a special emphasis for Nequals6. Furthermore, the design of the DNA solution space will be presented, which is symbolized by a set of bit-strings (words).
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