Ajania purpurea Shih is a plant of the genus Ajania endemic to Tibet. As an ornamental plant, it has not been investigated for its pharmacological activity. In this study, HPLC analysis of 70% ethanol extracts of A. purpurea (EAPS) identified two major compounds, chlorogenic acid and piperine. We evaluated the cytoprotective activity and antioxidant effects of EAPS by examining the production of oxidative-stress-related mediators, including ROS, superoxide dismutase, and catalase, in glutamate-induced HT22 cells. JC-1 and TUNEL staining revealed that EAPS attenuated intracellular mitochondrial depolarization and subsequent apoptotic protein expression. Most importantly, EAPS promoted the nuclear translocation of Nrf2 and deliverance of HO-1 in HT22 cells. EAPS also inhibited glutamate-induced cytotoxicity and ROS production, which was partly changed by siRNA HO-1. These results intimate the effective cytoprotective and neuroprotective vitality of A. purpurea, peradventure via the adjustment of the Nrf2/HO-1 signaling pathway.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.