Photothermal PCR, which exploits the photothermal conversion effect based on the plasmonic absorption resonance of metal nanoparticles, has garnered significant attention for its capacity to enable rapid diagnosis. Through the addition of gold nanoshells, known for their high photothermal conversion efficiency in the near-infrared region, to the PCR mixture, we achieved photothermal quantitative PCR while simultaneously conducting fluorescence measurements during the photothermal PCR cycle. Our photothermal quantitative PCR method expeditiously amplifies Lambda DNA while preserving its detection sensitivity, successfully amplifying the target DNA within just 25 minutes and detecting a minimum of 50 picograms of DNA. Furthermore, we demonstrated the versatility of photothermal quantitative PCR by applying it to genomic DNA extracted from Salmonella, showcasing its effectiveness with long base pairs. By leveraging the photothermal properties of gold nanoshells, our innovative approach to photothermal qPCR will pave the way for point-of-care diagnostics of nucleic acid biomarkers.
The global rise of infectious diseases underscores the urgent need for rapid, accurate diagnostics. Our study introduces a novel polymerase chain reaction technique utilizing gold nanoshells for faster and more efficient DNA amplification. These nanoshells' unique properties enable rapid heating in the near-infrared spectrum, accelerating the PCR process by quickly reaching optimal temperatures. This approach streamlines DNA amplification and ensures detection of small DNA quantities. Integrating photothermal PCR with advanced real-time fluorometry and non-invasive temperature monitoring, we can amplify DNA in just 25 minutes and detect as little as 50 picograms. The use of gold nanoshells' heating capabilities leads to quicker, more sensitive DNA detection. This innovation is a significant advancement in PCR technology, especially for point-of-care diagnostics, promising quick identification of DNA markers vital for prompt infectious disease diagnosis. It's particularly valuable in urgent care or resource-limited settings, where rapid, reliable results are essential.
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