Optical imaging of human midbrain organoids in the field of Parkinson research is challenging using current established technologies since they require lengthy and invasive clearing methods, which preclude live organoid observations and longitudinal studies.
Raster-Scanning Optoacoustic Mesoscopy (RSOM) overcomes this limitation by utilizing the intrinsic optical absorption contrast based on the optoacoustic effect. We show that RSOM is able to provide fast quantitative visualization of the neuromelanin content in intact large organoids at single cell resolution.
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