The aim of this study was to develop a microfluidic-powered optical platform to real-time monitor microbial biofilm formation at the single-cell level under a precisely controlled laminar flow condition and to rapidly evaluate the cleaning effect of laser irradiation on a mature microbial biofilm. A 405-nm laser light was used to evaluate the cleaning effects on both mature biofilms. The results show that Staphylococcus aureus biofilm was reduced by 80% in population, which was 20% higher than that of Candida albicans biofilm. A further study will be conducted in a poly-microbial biofilm commonly causing urinary tract infections.
KEYWORDS: Microfluidics, Bacteria, Laser therapeutics, Visualization, Systems modeling, Process modeling, Modulation, Microscopes, Medicine, Medical research
Microfluidics technology holds a great promise in evaluating the biofilm growth and the effectiveness of real-time cleaning. We have developed a microfluidic-assisted optical system to evaluate the cleaning effect of bacteria using lasers.
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