A 3D centrifugal cartridge with gravity valve, capillary valves, and Coriolis switching valve was designed, fabricated, and tested for Solid Phase Extraction (SPE). In particular, the novel three-dimensional gravity valve helps to release reagents in not-sequentially increased rotation speeds. The sequential control of these valves was simply achieved by manipulating the rotating direction and speed of the cartridge. The prototype microfluidic cartridge was 3D printed with a FDM printer and experimentally tested. Anthracene, which was one of the Polycyclic Aromatic Hydrocarbons (PAHs), was used as the target analyte and tested. C18 was used as the stationary sorbent and fluorescence method was adopted to detect anthracene. The concentrations of the anthracene testing samples were 1μg/L (1ppb), 5μg/L, and 10μg/L (anthracene/water), respectively. The volumes of testing sample and eluent (nonane) were 10mL and 1mL respectively. Experiments showed that 7 times of enrichment of the original samples were achieved. This centrifugal SPE system could be potentially used as a portable on-situ pollutant enrichment and detection device.
Solid phase extraction (SPE) is widely used for pre-concentration of the target molecules in the liquid sample to realize a lower detection limit of the target molecules. Fluorescent detection often follows the SPE process to determine the existence and concentration of these molecules. However, conventional SPE process are complex and time consuming. We present a novel centrifugal microfluidic platform based on 3D printing technology in this paper. Specially designed mechanical pinch valves, which were controlled by the rotation frequency of the platform, were used to manipulate the flow of the reagent and fluid sample. The prototype of the proposed system was fabricated with in 3D printing technology. The platform was tested with 10ppm (part per million) standard oil-water mixing sample. Different stationary sorbent, such as C18, activated charcoal, and 3D printable porous polymer, were used and compared. The experimental results showed that the fluorescence intensity of the sample was significantly increased. This SPE platform is easy to operate and can be potentially used as a portable on-situ spilled oil enrichment and detection device. Other than crude oil, this platform can be also used for other pollutant in environment water.
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