Ms. Na-rae Jo Profile

Na-rae Jo

at Korea Research Institute of Bioscience and Biotech

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Publications (2)

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Proceedings Article | 15 July 2015 Paper

Ultra-sensitive detection of biomarker using localized surface plasmon resonance (LSPR) enhanced by ELISA

Yong-Beom Shin, Na rae Jo, Ki joong Lee

Proceedings Volume 9537, 95371F (2015) https://doi.org/10.1117/12.2183063

KEYWORDS: Gold, Metals, Nanoimprint lithography, Surface plasmons, Resonance enhancement, Ultraviolet radiation, Glasses, Biosensing, Biosensors, Semiconducting wafers

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We demonstrate a highly sensitive detection of AFP (α-fetoprotein) protein (liver cancer marker) in human serum using the LSPR biosensor. Gold metal nanodot array (MNA) on a glass wafer were fabricated by UV nanoimprint lithography (NIL). After the NIL process using a film stamp and the removal of residual layer via oxygen plasma etching, metal films were deposited using an electron-beam evaporator, followed by the lift-off step. Consequently, the gold MNA was realized on 5-inch glass wafer and the pitch, diameter and height of MNA were 300nm, 150 nm and 20 nm, respectively. We employed observation of LSPR spectra via back-reflection, which provides a stable measurement of LSPR because a probe light does not pass a bio-sample. In addition, one channel among two flow channels was used a control channel, the MNA surface in which was modified with bovine serum albumin, not antibody. After antigen-antibody reaction, the enzyme/precipitation was employed on the MNA (Nano-ELISA). As a result, we could detect AFP in 50L human serum with limit of detection (LOD) of 0.7 zeptomole (10^-21 mole).

Proceedings Article | 8 May 2014 Paper

Nanoplasmonic biosensor for cancer diagnosis: design and fabrication

Yong-Beom Shin, Na-rae Cho, Ki Joong Lee

Proceedings Volume 9129, 91292M (2014) https://doi.org/10.1117/12.2051585

KEYWORDS: Cancer, Nanoplasmonics, Gold, Nanoimprint lithography, Biosensors, Metals, Sensors, Glasses, Semiconducting wafers, Nanolithography

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In this study, gold nanoplasmonic biosensors using localized surface plasmon resonance (LSPR) were fabricated for the diagnosis of cancer. We optimized the structures of the gold nanodot array (GNA) via the experiments for the optical characteristics. In addition, the nanoimprint lithography was employed for realizing nanoplasmonic structures, which is a more efficient technique for mass production than nanolithography such as electron beam lithography (EBL) or focused ion beam (FIB) lithography that is a quite intricate, time-consuming and expensive process.
After the UV nanoimprinting process using a film stamp and metal films were deposited using an electron-beam evaporator, followed by the lift-off step. Consequently, the nanoplasmonic MNA was realized on 5-inch glass wafer and the pitch, diameter and height of MNA were 300nm, 150 nm and 20 nm, respectively. The wavelength of nanoplasmonic resonance peak represented from the MNA sensors was about 740nm under aqueous ambient.
The capture antibodies of the lung and the pancreas cancer marker, respectively, were immobilized on the surfaces of MNA sensor. Using a compact fiber-optic spectrometer and a reflection optical probe, we were able to confirm the binding of cancer markers with their antibodies due to the immunoreactions between each cancer marker and its corresponding antibody on the sensor surfaces. The amount of the cancer markers in serum were analyzed through the observation of nanoplasmonic resonance wavelength-shift on the reflection spectra. To amplify a sensitivity of detection demonstrated by the nanoplasmonic resonance peak shift, we applied enzyme-precipitation reaction on the surface of MNA biosensor. The enzyme-catalyzed precipitation method in the GNA biosensor could be extended to detect other clinical biomarkers at extremely low concentrations in actual clinical samples.

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