The image quality assessment is the basis of autofocus for monoclonal-picking instrument. For the random distribution of the monoclonal colonies, the following situation may happen, edge blurring or edge overlapping. In practice, due to the complicated morphological characteristics of colonies, to a certain degree, camera takes image with feeble features is unavoidable, so the image quality assessment based on the image edge for monoclonal-picking instrument is proposed in this paper. The experimental results show that when dealing with the image of fine colony or image with feeble features, this method is consistent with fast autofocus demand, its accuracy is higher and consumes less storage.
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