Ms. Dana Wegierak Profile

Dana Wegierak

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Publications (3)

Proceedings Article | 13 March 2024 Poster

Rational design of ICG-based contrast agents for near-infrared photoacoustic imaging

Marzieh Hanafi, Nicholas Such, Giovanni Giammanco, Shrishti Singh, Dana Wegierak, Eric Abenojar, Pinunta Nittayacharn, Tessa Kosmides, Agata Exner, Remi Veneziano, Parag Chitnis

Proceedings Volume PC12842, PC1284220 (2024) https://doi.org/10.1117/12.3003260

KEYWORDS: Contrast agents, Photoacoustic imaging, Design and modelling, Nanoprobes, In vivo imaging, Fluorescence imaging, Fluorescence, Dyes, Absorption, Whole blood

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Proceedings Article | 12 March 2024 Poster + Paper

The use of speckle decorrelation OCT to detect nanobubbles in cell pellet aggregates

Arash Javanmardi, Elizabeth Berndl, Dana Wegierak, Agata Exner, Michael Kolios

Proceedings Volume 12856, 1285605 (2024) https://doi.org/10.1117/12.3002019

KEYWORDS: Optical coherence tomography, Speckle, Signal to noise ratio, Ultrasonography, Autocorrelation, Nanobubbles, Confocal microscopy, Signal intensity

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SPIE Journal Paper | 21 January 2022 Open Access

Effects of shell-integrated Sudan Black dye on the acoustic activity and ultrasound imaging properties of lipid-shelled nanoscale ultrasound contrast agents

Dana Wegierak, Grace Fishbein, Eric Abenojar, Al Christopher De Leon, Jinle Zhu, Yanjie Wang, Charlotte Ferworn, Agata Exner, Michael Kolios

JBO, Vol. 27, Issue 01, 016501, (January 2022) https://doi.org/10.1117/12.10.1117/1.JBO.27.1.016501

KEYWORDS: Niobium, Signal detection, Acoustics, Magnesium, Transducers, Nonlinear optics, Ultrasonography, Absorption, Transmission electron microscopy, Particles

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Significance: An effective contrast agent for concurrent multimodal photoacoustic (PA) and ultrasound (US) imaging must have both high optical absorption and high echogenicity. Integrating a highly absorbing dye into the lipid shell of gas core nanobubbles (NBs) adds PA contrast to existing US contrast agents but may impact agent ultrasonic response. Aim: We report on the development and ultrasonic characterization of lipid-shell stabilized C3F8 NBs with integrated Sudan Black (SB) B dye in the shell as dual-modal PA-US contrast agents. Approach: Perfluoropropane NBs stabilized with a lipid shell including increasing concentrations of SB B dye were formulated by amalgamation (SBNBs). Physical properties of SBNBs were characterized using resonant mass measurement, transmission electron microscopy and pendant drop tensiometry. Concentrated bubble solutions were imaged for 8 min to assess signal decay. Diluted bubble solutions were stimulated by a focused transducer to determine the response of individual NBs to long cycle (30 cycle) US. For assessment of simultaneous multimodal contrast, bulk populations of SBNBs were imaged using a PA and US imaging platform. Results: We produced high agent yield (∼1011) with a mean diameter of ∼200 to 300 nm depending on SB loading. A 40% decrease in bubble yield was measured for solutions with 0.3 and 0.4 mg / ml SB. The addition of SB to the shell did not substantially affect NB size despite an increase in surface tension by up to 8 mN / m. The bubble decay rate increased after prolonged exposure (8 min) by dyed bubbles in comparison to their undyed counterparts (2.5-fold). SB in bubble shells increased gas exchange across the shell for long cycle US. PA imaging of these agents showed an increase in power (up to 10 dB) with increasing dye. Conclusions: We added PA contrast function to NBs. The addition of SB increased gas exchange across the NB shell. This has important implications in their use as multimodal agents.

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