Mr. Zhang Geng Profile

Zhang Geng

at Tokyo Medical and Dental Univ.

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Proceedings Article | 22 May 2023 Presentation + Paper

Acetone vapor imaging using secondary alcohol dehydrogenase immobilized on a laser-patterned hydrophilic PTFE mesh

Kenta Iitani, Naoki Mizukoshi, Zhang Geng, Kohji Mitsubayashi

Proceedings Volume 12327, 1232708 (2023) https://doi.org/10.1117/12.2657101

KEYWORDS: Acetone, Imaging systems, Nicotinamide adenine dinucleotide, Mode conditioning cables, Fluorescence, Fluorescence intensity, Fluorescence imaging, Tunable filters, Optical filters, Gas sensors

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Trace acetone contained in the human-derived vapor is a potential indicator of lipid metabolisms. Exhaled breath and skin gas contain various kinds of volatile chemicals. Thus, major analytical systems implement chromatographical separation techniques to measure only target chemicals without interference. Such systems are suitable for clinical lab analysis but not for monitoring personal health status. Optical biosensors are suitable for measuring human-derived vapor in a real-time manner because of their high selectivity and sensitivity. Here we report an acetone vapor imaging technique based on secondary alcohol dehydrogenase (S-ADH) immobilized on a laser-patterned hydrophilic polytetrafluoroethylene (PTFE) mesh. The S-ADH catalyzes the reduction of acetone to 2-propanol with concurrent oxidation of coenzyme nicotinamide adenine dinucleotide (NADH) to NAD+. The NADH can be excited by 340 nm of ultraviolet light and emits visible fluorescence at around 490 nm. Acetone concentration can be transduced to a decrement in fluorescence intensity by combining the NADH fluorescence property and S-ADH reaction. S-ADH was immobilized on a breathable hydrophilic PTFE mesh fabricated with laser patterning. In the experiment, fluorescence intensity distribution changing by application of acetone vapor to the S-ADH immobilized mesh was captured by the camera. Fluorescence intensity rapidly decreased at the application point. The decrement was correlated to acetone vapor concentration. The high selectivity against acetone was also observed at typical concentrations and components of volatiles in human breath. Those characteristics will enable the developed technique to measure acetone in human samples in the future.

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