The imaging speed of current mid-infrared photothermal (MIP) microscope is limited to tens of seconds per frame due to the long pixel dwell time and slow sample scanning process, which is insufficient for capturing dynamics inside living systems. In this work, we developed a video-rate MIP microscope by employing a lock-in free demodulation scheme to resolve single IR pulse induced contrast. We further developed a synchronous pump-probe Galvo scanning for reaching a line rate over 2kHz. With such scheme, the system is capable of resolving chemical dynamics of various biomolecules in living organisms at multiple scales.
KEYWORDS: In vivo imaging, Mid-IR, Cancer, Signal generators, Photoacoustic spectroscopy, Magnetic resonance imaging, Infrared imaging, In vitro testing, Image resolution, Absorbance
Enzymes are vital in most physiological and biochemical processes and may function as critical biomarkers of disease. Notably, many biological events and signaling networks involve multiplex enzyme species. Thus, mapping multiple enzyme activities is significant for elucidating enzymatic functions in life and disease. Here, we report a novel technology to map the catalytic efficacy of phosphatase and caspase in live cancer cells and in C. elegans by mid-infrared photothermal (MIP) imaging of nitrile chameleons. This technology will certainly bring new insight into the roles of enzyme in biochemical activities and explore new knowledge of enzymatic activity in health and disease.
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