KEYWORDS: In vivo imaging, Mid-IR, Cancer, Signal generators, Photoacoustic spectroscopy, Magnetic resonance imaging, Infrared imaging, In vitro testing, Image resolution, Absorbance
Enzymes are vital in most physiological and biochemical processes and may function as critical biomarkers of disease. Notably, many biological events and signaling networks involve multiplex enzyme species. Thus, mapping multiple enzyme activities is significant for elucidating enzymatic functions in life and disease. Here, we report a novel technology to map the catalytic efficacy of phosphatase and caspase in live cancer cells and in C. elegans by mid-infrared photothermal (MIP) imaging of nitrile chameleons. This technology will certainly bring new insight into the roles of enzyme in biochemical activities and explore new knowledge of enzymatic activity in health and disease.
Optical coherence tomography (OCT) has been a powerful 3D optical imaging tool in the last decade while it lacks molecular information. In this work, we integrate the mid-infrared photothermal microscopy with the OCT approach to demonstrate a bond-selective full-field optical coherence tomography (BS-FF-OCT), in which a pulsed mid-infrared laser is used to modulate the full-field OCT signal through the photothermal effect. This method achieves label-free volumetric infrared spectroscopic imaging at 1-μm isotropic resolution, demonstrated by a variety of samples, including 1 μm PMMA beads embedded in agarose gel, polypropylene fiber mattress, myelinated nerve bundle in mouse brain tissue, Caenorhabditis elegans, and cancer cell spheroids.
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