The detection and imaging of the microvascular map play a vital role in providing valuable pathological evidence for a variety of physiological disorders. Optical coherence tomography angiography (OCTA) is a non-invasive technique that allows high-speed, high-resolution visualization of microvascular networks without the need for contrast agents and invasive manipulation. In this study, we developed a fiber-based polarization-sensitive optical coherence tomography (PS-OCT) system that utilizes a 200 kHz swept source at 1310 nm. To reduce phase artifacts and provide excellent motion contrast for vascular imaging, we have incorporated the complex correlated phase gradient variance (CCPGV) method. Additionally, to address the challenges posed by birefringence dispersion, we implemented a dual-state numerical dispersion compensation method. By imaging human skin, we have successfully demonstrated the effectiveness of our system in visualizing the vascular map while minimizing noise interference. The high-quality imaging and accurate visualization of vascular networks provided by our system hold significant potential in enhancing the understanding and assessment of various pathological conditions. This can benefit both researchers and clinicians in their biomedical investigations and patient care.
Optical coherence tomography (OCT) is a new imaging technique for observing biological tissue structure. Polarization-sensitive optical coherence tomography (PS-OCT) is a functional extension of OCT, which uses polarized light to probe the sample and extract the polarization properties by detecting the change of the polarization state. In the practical application environment, the optical characteristics of biological samples and the instability of the system will reduce the contrast of the image. Traditional contrast enhancement methods are mostly based on histogram equalization and histogram matching to improve the global quality, which causes the loss of some details of the image and the excessive enhancement of noise. To solve this problem, a local contrast enhancement method is proposed in this paper. The target region and the background region are processed differently by threshold segmentation to enhance the contrast of the signal region and restrain the excessive enhancement of the background noise at the same time. The experimental results showed that this method can enhance the contrast of PS-OCT images and improve the image quality effectively. We believe that this method may play an important role in the application of PS-OCT technology in clinical research.
In order to analyze the polarization properties of biological tissues, we constructed a high-speed three-dimensional swept -source polarization sensitive optical coherence tomography (PS-OCT) imaging system with the axial scanning rate up to 200KHz. For automatically detecting and analyzing the polarization properties of peristaltic living tissues in real-time, an advanced measurement and control system is designed using laboratory virtual instrument technology. Based on the producer-consumer pattern, the system mainly includes the sample arm scanning galvanometer module, the interference data acquisition module, the interference data real-time processing and display module, the interference data storage module. Each module not only independently completes specific sub-functions but also interacts with others. In-vivo human finger and in-vitro pork tissue were imaged using the system. The experimental results show that the home-made system obtains 78 cross sectional B-scans per second, and can display two-dimensional OCT images and acquire threedimensional OCT data all in real-time. This robust measurement and control system has the characteristics of short design cycle, high flexibility and real-time monitoring, will significantly promote the development of PS-OCT in clinical applications.
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