The basics for investigating histological brain tissue sections using polarization and differential Mueller matrix microscopy are provided. The structural and logical scheme, design, and examples of results for the Stokes polarimetric mapping technique of microscopic images of deceased brain tissue sections are presented, where orientation (OP) and phase (FP) parameters are determined. Furthermore, the structural and logical scheme, design, and examples of results for the azimuthally-invariant Mueller matrix mapping technique of histological brain tissue sections from deceased individuals are presented, where optical activity (MMI OA) and linear dichroism (MMI LD) maps are determined.
The structural-logical diagram and research design by the methods of polarization-phase tomography of linear dichroism of the polycrystalline structure [1-5] of histological sections of the brain are presented. Differential diagnosis of the formation of hemorrhages of traumatic genesis, cerebral infarction of ischemic and hemorrhagic genesis by the method of differential Mueller-matrix mapping of amplitude anisotropy - linear dichroism maps (ALD) of histological brain sections and operational characteristics of the method of their statistical analysis. Differential diagnosis of the prescription of the formation of hemorrhages of traumatic genesis, cerebral infarction, ischemic and hemorrhagic genesis by the method of differential Mueller-matrix mapping of amplitude anisotropy - temporal dynamics of changes in the statistical structure of ALD maps of histological brain sections.
The paper presents the structural-logical diagram and research design of the newest method of 3D Mueller-matrix microscopy of the layer-by-layer structure of the polycrystalline component [1-9] of depolarizing histological sections of the brain. Principles of differential diagnosis of the formation of hemorrhages of traumatic genesis, cerebral infarction ischemic and hemorrhagic genesis by the method of 3D Mueller-matrix microscopy. Layer-by-layer azimuthal-invariant Mueller-matrix images of circular birefringence (MMI OA) of histological brain sections and operational characteristics of the method of their statistical analysis were determined.
The materials of the experimental determination of the set of maps and histograms of the distribution of the magnitude of the fluctuations of linear (DFLB) birefringence for differential diagnosis and the formation of hemorrhages of traumatic origin, cerebral infarction ischemic and hemorrhagic genesis by diffuse tomography of the polycrystalline structure of histological sections of the brain.
1. Development of a structural-logical scheme and experimental testing of methods and means of diffuse tomography new in forensic practice for reproducing fluctuations in the magnitude of linear and circular birefringence and dichroism of the polycrystalline structure of histological sections of the brain. 2. Experimental determination of a set of maps and histograms fluctuation distributions of linear (FFLB) and circular (FFCB) birefringence and dichroism (FALD and FACD) for differential diagnosis of traumatic hemorrhage, cerebral infarction ischemic and hemorrhagic genesis using diffuse tomography of polycrystalline structure of histological sections of the brain. 3. Information analysis to determine the operational characteristics of the force (sensitivity, specificity and balanced accuracy) of the diffuse tomography method.
The report contains a structural-logical diagram and design of studies using polarization and Mueller-matrix microscopy methods of histological sections of the brain. The results of the differential diagnosis of the formation of hemorrhages of traumatic origin, ischemic cerebral infarction and hemorrhagic genesis using Mueller-matrix microscopy are presented.
Azimuthally invariant Mueller-matrix images of linear birefringence (MMI LB) of histological sections of the brain and operational characteristics of the method of their statistical analysis.
Azimuthally invariant Mueller-matrix images of circular birefringence (MMI CB) of histological sections of the brain and operational characteristics of the method of their statistical analysis
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Differential diagnosis of the duration of the formation of hemorrhages of traumatic origin, ischemic cerebral infarction and hemorrhagic genesis using Mueller matrix mapping.
Temporal dynamics of changes in the statistical structure of maps of Mueller-matrix invariants of optical activity (MMI OA) of histological sections of the brain.
Temporal dynamics of changes in the statistical structure of maps of Mueller-matrix invariants of linear birefringence (MMI LB) of histological sections of the brain.
By Mueller-matrix mapping of changes in the distribution of the magnitude of matrix invariants characterizing the degree of crystallization of histological sections of the brain, liver and kidney, the forensic medical criteria were determined and the interval for determining the damage duration of 24 hours with an accuracy of 45min. - 50 min. was established. For azimuthally invariant polarization mapping of changes in the distributions of Mueller-matrix invariants characterizing the optical activity of molecular complexes of histological sections of the brain, liver, and kidney, forensic medical criteria were developed for the first time and accuracy was improved to 35 min. - 40 min. in the interval for determining the damage duration period of 72 hours. For highly accurate objective determination of the damage duration in a long time interval (1 hour - 120 hours), a polarization tomography method has been developed (reproducing the distributions of birefringence of fibrillar networks of histological sections of internal organs), which provides an accuracy of 25 minutes. (1 hour - 24 hours) to 45 minutes (24 hours - 120 hours).
For a high-precision objective histological determination of the prescription of damage to internal organs over a long period of time, a systematic approach was used based on digital azimuthally invariant polarizing, Mueller-matrix and tomographic methods for studying temporary changes in the molecular and polycrystalline structure of brain, liver and kidney samples in the post-mortal period. It was revealed that a linear change in the magnitude of statistical moments of the 1st - 4th orders characterizing the distribution of data of digital azimuthally invariant polarizing, Mueller-matrix and tomographic methods is interconnected with the duration of damage to internal organs. On this basis, a new algorithm for digital histological determination of the prescription of the occurrence of damage is proposed. To determine the extent of damage, the method of azimuthally invariant polarization microscopy with different magnification of the image of histological sections of tissues of internal organs was used, which provided diagnostic relationships between changes in the magnitude of statistical moments of the 1st to 4th orders, which characterize the azimuth and elliptic polarization maps of digital microscopic images and time intervals of damage duration.
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