Dr. Mihaela Balu Profile

Dr. Mihaela Balu

Research Associate Professor at Beckman Laser Institute and Medical Clinic

SPIE Involvement:

Conference Program Committee | Editorial Board Member: Biophotonics Discovery | Author

Publications (26)

SPIE Journal Paper | 17 December 2024 Open Access

Collagen remodeling in murine melanoma therapy response through second-harmonic generation imaging

Mihaela Balu

BIOS, Vol. 1, Issue 03, 030501, (December 2024) https://doi.org/10.1117/12.10.1117/1.BIOS.1.3.030501

KEYWORDS: Collagen, Melanoma, Second harmonic generation, Tumors, Biological imaging, Cancer, Animal model studies, Optical imaging, In vivo imaging, Visualization

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Proceedings Article | 17 March 2023 Presentation

Label free multi-photon metabolic imaging: from mitochondria to humans

Irene Georgakoudi, Christopher Polleys, Yang Zhang, Volha Liaudanskaya, Adriana Sanchez, Einstein Gnanatheepam, Maria Savvidou, David Kaplan, Hong-Thao Thieu, Elizabeth Genega, Thomas Schenlldorfer, Jessica Shiu, Anand Ganesan, Mihaela Balu

Proceedings Volume PC12363, PC123630G (2023) https://doi.org/10.1117/12.2658901

KEYWORDS: Tissues, Traumatic brain injury, Statistical modeling, Nondestructive evaluation, Luminescence, In vivo imaging

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Proceedings Article | 15 March 2023 Presentation

Intravital optical imaging of wound healing in human skin (Conference Presentation)

Mihaela Balu, Juvinch Vicente, Amanda Durkin, Kristen Kelly, Jessica Shiu, Anand Ganesan

Proceedings Volume PC12384, PC1238404 (2023) https://doi.org/10.1117/12.2648732

KEYWORDS: Wound healing, Skin, Optical imaging, Optical tracking, In vivo imaging, Visualization, Tissues, Tissue optics, Resolution enhancement technologies, Optical fibers

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Proceedings Article | 24 May 2022 Presentation

Development of a fast large area multiphoton exoscope (FLAME) for clinical skin imaging

Juvinch Vicente, Kristina Shrestha, Amanda Durkin, Mihaela Balu

Proceedings Volume PC12146, PC121460I (2022) https://doi.org/10.1117/12.2621451

KEYWORDS: Skin, Image resolution, Second-harmonic generation, Luminescence, Image restoration, Collagen, Visualization, Tissue optics, Statistical modeling, Signal to noise ratio

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Proceedings Article | 7 March 2022 Presentation

Laser photodamage threshold assessment for human skin imaging by multiphoton microscopy

Kristina Shrestha, Chung-Ho Sun, Amanda Durkin, Mihaela Balu

Proceedings Volume PC11934, PC119340J (2022) https://doi.org/10.1117/12.2610054

KEYWORDS: Skin, Multiphoton microscopy, Laser damage threshold, In vivo imaging, Laser safety, Signal generators, Second-harmonic generation, Optical design, Luminescence, Image resolution

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Proceedings Article | 7 March 2022 Presentation

Fast large area multiphoton exoscope (FLAME) for improving the detection accuracy of early melanoma

Mihaela Balu, Juvinch Vicente, Kristina Shrestha, Amanda Durkin, Christopher Zachary, Anand Ganesan, Kristen Kelly

Proceedings Volume PC11944, PC1194405 (2022) https://doi.org/10.1117/12.2617030

KEYWORDS: Melanoma, Skin, Visualization, Collagen, Tissues, Tissue optics, Signal generators, Signal detection, Resolution enhancement technologies, Optical fibers

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Proceedings Article | 7 March 2022 Presentation

Enhancement of the cutaneous melanin assessment in human skin by in vivo imaging using fast large-area multiphoton exoscope (FLAME)

Juvinch Vicente, Griffin Lentsch, Amanda Durkin, Mihaela Balu

Proceedings Volume PC11944, PC119440J (2022) https://doi.org/10.1117/12.2610037

KEYWORDS: Skin, In vivo imaging, Luminescence, Tissues, Resolution enhancement technologies, Multiphoton microscopy, Melanoma, Image resolution

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Proceedings Article | 5 March 2021 Open Access

Non-invasive, label-free, two-photon detection of epithelial metabolic precursors to repigmentation in vitiligo micrografts

Christopher Polleys, Griffin Lentsch, Bruce Tromberg, Anand Ganesan, Francisca Rius-Diaz, Mihaela Balu, Irene Georgakoudi

Proceedings Volume 11655, 116550F (2021) https://doi.org/10.1117/12.2578676

KEYWORDS: Skin, Luminescence, Tissues, Phototherapy, Multiphoton processes, Multiphoton microscopy

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Proceedings Article | 5 March 2021 Presentation

Development of a fast, large area multiphoton exoscope (FLAME) for clinical skin imaging

Mihaela Balu, Alexander Fast, Amanda Durkin, Griffin Lentsch

Proceedings Volume 11654, 116540C (2021) https://doi.org/10.1117/12.2582795

KEYWORDS: Skin, Tissues, Image resolution, Imaging systems, Diagnostics, Tissue optics, Time resolved spectroscopy, Spatial resolution, Single photon, Multiphoton microscopy

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Proceedings Article | 5 March 2021 Presentation

Mapping out lesion heterogeneity on (sub)cm scale with a fast large area multiphoton exoscope (FLAME)

Alexander Fast, Griffin Lentsch, Amanda Durkin, Christopher Zachary, Kristen Kelly, Mihaela Balu, Linda Doan, Bonnie Lee

Proceedings Volume 11618, 116180X (2021) https://doi.org/10.1117/12.2579260

KEYWORDS: Skin, In vivo imaging, Associative arrays, Tumors, Melanoma, Image resolution, Diagnostics, Denoising, Convolutional neural networks, Chemical elements

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Proceedings Article | 5 March 2021 Open Access

In-vivo optical microscopy and single cell transcriptomics approaches provide insights into therapeutic response of vitiligo

Griffin Lentsch, Jessica Shiu, Jessica Flesher, Pezhman Mobasher, Christopher Polleys, Craig Mizzoni, Karsten König, Suoqin Jin, Lihua Zhang, Bruce Tromberg, Qing Nie, Irene Georgakoudi, Anand Ganesan, Mihaela Balu

Proceedings Volume 11618, 116180P (2021) https://doi.org/10.1117/12.2582167

KEYWORDS: Skin, In vivo imaging, Optical microscopy, Resistance, Reflectivity, Quantitative analysis, Multiphoton microscopy, Confocal microscopy

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Proceedings Article | 6 March 2020 Open Access

In vivo optical imaging of vitiligo skin grafting treatment using multiphoton microscopy and reflectance confocal microscopy (Conference Presentation)

Griffin Lentsch, Pezhman Mobasher, Craig Mizzoni, Karsten Koenig, Bruce Tromberg, Irene Georgakoudi, Anand Ganesan, Mihaela Balu

Proceedings Volume 11211, 112110O (2020) https://doi.org/10.1117/12.2544056

KEYWORDS: Skin, In vivo imaging, Multiphoton microscopy, Reflectivity, Confocal microscopy, Optical imaging, Phototherapy, Wound healing, Laser optics, Tissue optics

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Proceedings Article | 6 March 2020 Presentation

High-speed, high-resolution mesoscopic multiphoton microscopy of human skin (Conference Presentation)

Alexander Fast, Anand Ganesan, Kristen Kelly, Christopher Zachary, Mihaela Balu

Proceedings Volume 11211, 112110F (2020) https://doi.org/10.1117/12.2543603

KEYWORDS: Skin, Multiphoton microscopy, Tissues, Image resolution, Skin cancer, Harmonic generation, Second-harmonic generation, Luminescence, Clinical research, Melanoma

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Proceedings Article | 4 March 2019 Open Access

In vivo multiphoton microscopy imaging of vitiligo (Conference Presentation)

Griffin Lentsch, Mihaela Balu, Karsten Koenig, Seongjin Jo, Bruce Tromberg, Anand Ganesan

Proceedings Volume 10851, 108510T (2019) https://doi.org/10.1117/12.2508791

KEYWORDS: Skin, Multiphoton microscopy, In vivo imaging, Phototherapy, Visualization, Optical imaging, Laser scanners, Microscopy, Molecular lasers, Luminescence

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Proceedings Article | 14 March 2018 Open Access

Feature characterization of scarring and non-scarring types of alopecia by multiphoton microscopy (Conference Presentation)

Inga Saknite, Manuel Valdebran, Jessica Lin, Griffin Lentsch, Joshua Williams, Mihaela Balu, Bruce Tromberg, Natasha Mesinkovska

Proceedings Volume 10467, 104670I (2018) https://doi.org/10.1117/12.2291410

KEYWORDS: Multiphoton microscopy, Luminescence, Harmonic generation, Biopsy, Two photon imaging, Visualization, In vivo imaging, Second-harmonic generation, Collagen, Quantitative analysis

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Proceedings Article | 1 May 2017 Open Access

Bio-optic signatures for advanced glycation end products in the skin in streptozotocin (STZ) Induced Diabetes (Conference Presentation)

Mayer Saidian, Adrien Ponticorvo, Rebecca Rowland, Melisa Balbado, Griffin Lentsch, Mihaela Balu, Micheal Alexander, Li Shiri, Jonathan R. Lakey, Anthony Durkin, Roni Kohen, Bruce Tromberg

Proceedings Volume 10060, 1006014 (2017) https://doi.org/10.1117/12.2251278

KEYWORDS: Medical laser equipment, Nerve, Multiphoton microscopy, Biopsy, Collagen, Skin, Blood, Glucose, Proteins, Tissues

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Proceedings Article | 19 April 2017 Open Access

In-vivo multiphoton microscopy (MPM) of laser-induced optical breakdown (LIOB) in human skin (Conference Presentation)

Mihaela Balu, Griffin Lentsch, Dorota Korta, Karsten Konig, Kristen Kelly, Bruce Tromberg, Christopher Zachary

Proceedings Volume 10037, 100370F (2017) https://doi.org/10.1117/12.2253566

KEYWORDS: Medical laser equipment, Signal attenuation, Skin, Laser optics, In vivo imaging, Tissue optics, Multiphoton microscopy, Microscopes, Picosecond phenomena, Nd:YAG lasers

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Proceedings Article | 27 April 2016 Open Access

Comparing Yb-fiber and Ti:Sapphire lasers for depth resolved imaging of human skin (Conference Presentation)

Mihaela Balu, Ilyas Saytashev, Jue Hou, Marcos Dantus, Bruce Tromberg

Proceedings Volume 9689, 96890X (2016) https://doi.org/10.1117/12.2213352

KEYWORDS: Skin, Fiber lasers, Ytterbium, Sapphire lasers, Medical laser equipment, Multimodal imaging, Harmonic generation, Laser systems engineering, Diagnostics, Current controlled current source

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Proceedings Article | 14 March 2016 Open Access

Large field of view multiphoton microscopy of human skin

Mihaela Balu, Hideharu Mikami, Jue Hou, Eric Potma, Bruce Tromberg

Proceedings Volume 9712, 97121F (2016) https://doi.org/10.1117/12.2216163

KEYWORDS: Skin, Multiphoton microscopy, Two photon imaging, Microscopes, Beam expanders, Monochromatic aberrations, Two photon excitation microscopy, Tissues, Objectives, Imaging systems, Relays, Scanners, Wavefronts

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SPIE Journal Paper | 7 December 2015 Open Access

Sub‐40 fs, 1060‐nm Yb‐fiber laser enhances penetration depth in nonlinear optical microscopy of human skin

Mihaela Balu, Ilyas Saytashev, Jue Hou, Marcos Dantus, Bruce Tromberg

JBO, Vol. 20, Issue 12, 120501, (December 2015) https://doi.org/10.1117/12.10.1117/1.JBO.20.12.120501

KEYWORDS: Skin, Second-harmonic generation, Microscopy, Optical microscopy, Fiber lasers, Collagen, Multiphoton microscopy, Tissue optics, Sapphire lasers, Laser scattering

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SPIE Journal Paper | 12 June 2015 Open Access

In vivo measurements of cutaneous melanin across spatial scales: using multiphoton microscopy and spatial frequency domain spectroscopy

Rolf Saager, Mihaela Balu, Viera Crosignani, Ata Sharif, Anthony Durkin, Kristen Kelly, Bruce Tromberg

JBO, Vol. 20, Issue 06, 066005, (June 2015) https://doi.org/10.1117/12.10.1117/1.JBO.20.6.066005

KEYWORDS: Skin, Tissue optics, In vivo imaging, Spectroscopy, Scattering, Absorption, Tissues, Multiphoton microscopy, Light scattering, Chromophores

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Proceedings Article | 22 February 2013 Open Access

Clinical studies of pigmented lesions in human skin by using a multiphoton tomograph

Mihaela Balu, Kristen Kelly, Christopher Zachary, Ronald Harris, Tatiana Krasieva, Karsten König, Bruce Tromberg

Proceedings Volume 8588, 858812 (2013) https://doi.org/10.1117/12.2004697

KEYWORDS: Melanoma, In vivo imaging, Skin, Multiphoton microscopy, Second-harmonic generation, Laser applications, Luminescence, Visualization, Collagen, Neodymium

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SPIE Journal Paper | 12 December 2012 Open Access

Two-photon excited fluorescence lifetime imaging and spectroscopy of melanins in vitro and in vivo

Tatiana Krasieva, Chiara Stringari, Feng Liu, Chung-Ho Sun, Yu Kong, Mihaela Balu, Frank Meyskens, Enrico Gratton, Bruce Tromberg

JBO, Vol. 18, Issue 03, 031107, (December 2012) https://doi.org/10.1117/12.10.1117/1.JBO.18.3.031107

KEYWORDS: Fluorescence lifetime imaging, Luminescence, Skin, In vivo imaging, Fluorescence spectroscopy, Imaging spectroscopy, Spectroscopy, In vitro testing, Melanoma, Microscopy

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Proceedings Article | 9 February 2012 Open Access

Two-photon excited fluorescence spectroscopy and imaging of melanin in vitro and in vivo

Tatiana Krasieva, Feng Liu, Chung-Ho Sun, Yu Kong, Mihaela Balu, Frank Meyskens, Bruce Tromberg

Proceedings Volume 8226, 82262S (2012) https://doi.org/10.1117/12.906490

KEYWORDS: Skin, In vivo imaging, Luminescence, Melanoma, Imaging spectroscopy, In vitro testing, Microscopy, Tissue optics, Fluorescence spectroscopy, Absorption

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Proceedings Article | 26 February 2010 Open Access

Scheme for efficient fiber-based CARS probe

Mihaela Balu, Gangjun Liu, Zhongping Chen, Bruce Tromberg, Eric Potma

Proceedings Volume 7569, 75693A (2010) https://doi.org/10.1117/12.855187

KEYWORDS: Tissues, CARS tomography, Signal detection, Single mode fibers, Femtosecond phenomena, Signal generators, Picosecond phenomena, Silica, In vivo imaging, Skin

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SPIE Journal Paper | 1 January 2009 Open Access

Effect of excitation wavelength on penetration depth in nonlinear optical microscopy of turbid media

Mihaela Balu, Tommaso Baldacchini, John Carter, Tatiana Krasieva, Ruben Zadoyan, Bruce Tromberg

JBO, Vol. 14, Issue 01, 010508, (January 2009) https://doi.org/10.1117/12.10.1117/1.3081544

KEYWORDS: Scattering, Second-harmonic generation, Microscopy, Photons, Tissues, Laser scattering, Optical microscopy, Luminescence, Collagen, Light scattering

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