We present an abundance analysis of six main sequence turnoff, subgiant, and giant branch stars toward the Galactic bulge that were observed with Keck/HIRES during microlensing events. This is an early look at the first detailed chemical analysis of main sequence stars in the Galactic bulge. Lensing events allow the effective aperture of Keck to be increased beyond its current dimensions; although, some events still stretched its spectroscopic capabilities. Future large telescopes with high resolution and high throughput spectrometers will allow the study of abundances in distant stellar populations and in less evolved stars with greater ease.
Parallel immunoassay enable simultaneous detection of many analytes forma small volume of sample fluid. The use of exceedingly small quantities of capture antibody immobilized in microspots facilitates ambient analyte assay conditions. Under these assy conditions, the fractional occupancy of capture antibody is independent of sample volume. Analyte concentrations are thus measured with greater sensitivity and potentially greater speed. The small drop size delivered by the Packard BioChip Arrayer, a non-contact piezoelectric robotic dispenser, enables the production of arrays consisting of high reproducible microspots of antibodies. The Packard HyrdoGel coated slide is a porous substrate based on a polymer matrix that provides a 3D hydrophilic environment similar to free solution suitable for biomolecular interactions. This substrate has been used to develop fluorescence-based detection immunoassay in multiplex with high sensitivity and offers advantages of increased probe capacity, functionality and accessability for other biomolecular screening applications. Images are captured with the ScanArray confocal laserscanner, which offers the use to choose from a wide range of fluorescent dyes with high resolution and sensitivity, and analyzed with QuantArray microarray analysis software, which enables researchers to easily and accurately visualize and quantitate gene expression and proteomics data.
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