Dr. Tingting Sun Profile

Dr. Tingting Sun

at Changchun Univ of Science and Technology

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 24 November 2021 Paper

Design simulation and application of integrated microarray chip based on polymerase chain reaction

Tingting Sun, Yuan Si, Yuanhua Yu

Proceedings Volume 12067, 1206703 (2021) https://doi.org/10.1117/12.2602860

Read Abstract +

Microarray chip is a highly sensitive and high-throughput genetic detection technology based on the principle of complementary pairing of nucleic acid sequence bases and using polymerase chain reaction (PCR), and its chip structure and material have a great impact on the detection. Therefore, a new type of PCR chip is designed. The large multi-physical field simulation software Comsol is used to simulate the coupling of thermodynamics and structural mechanics of the designed chip. Finite element simulation is used to experimentally optimize the chip material selection in practice, find the composite material (cyclic olefin copolymer (COC)) that has the least effect on signal processing and does not require polar treatment of the chip substrate, and design the optimal chip structure. Three kinds of standard chips (internal reference, negative and positive) were prepared by using FAM fluorescent probe. Positive luminescent spots were randomly labeled for detection. This detection is used to evaluate the value of practical application of this chip. The results show that the maximum deformation displacement of the chip is 0.6 mm at 4–100℃, which is instantaneously irradiated by a 1 KW radiation light source, and the transmittance of the model calculated by the penetrated radiation power is 83.7%. In practical application, several materials are irradiated with excitation light to detect their excitation effect, and COC has the smallest excitation effect and the lowest background signal value. A chip containing 203 microarray spots was prepared according to the above conclusions, and the detection results were 100% consistent.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years