Laser Speckle Contrast Imaging (LSCI) as the advantages of high speed, wide field of view, and ease of implementation has been widely used for imaging blood flow and vascular deformation during Vascular targeted Photodynamic Therapy (V-PDT). However, uncorrected direction and or scale kernel failed to improve the visualization of blood vessels derived from poor Signal Noise Ratio (SNR) of micro vessels during V-PDT. In this study, we extracted the adaptive scale and directional kernel using dual-wavelength reflection imaging to enhance laser speckle imaging. The in vivo data showed that the proposed method improved the 4%-29% performance of contrast-to-noise ratio (CNR) and provide a powerful monitoring tool for blood flow and vascular deformation during V-PDT.
Vascular-targeted photodynamic therapy (V-PDT) offers great promise as a treatment modality for vascular-related diseases. The injury of targeted blood vessels correlates to the singlet oxygen generation, which is affected by dosimetric parameters, including photosensitizer concentration, hemoglobin oxygenation concentration, and blood flow velocity. In this study, we developed an optical imaging system that combining hyperspectral imaging (HSI), dual-wavelength reflection imaging (DWRI) (λ1 = 500 nm and λ2 = 660 nm) and laser speckle imaging (LSI). The capability for monitoring dosimetric parameters has been demonstrated for in vivo imaging of hemoporfin-mediated V-PDT in a dorsal skinfold window chamber model. The HSI allows for simultaneously monitoring the changes of photosensitizer concentration and vasoconstriction of blood vessels, while the DWRI and LSI were used to measure hemoglobin oxygenation concentration and blood flow velocity, respectively. This study suggests that our home-built optical imaging system holds the potential for assessing the V-PDT efficiency in vivo and optimizing the treatment protocol.
Vascular targeted photodynamic therapy (V-PDT) has shown satisfied efficiency in treating vascular-related diseases including age-related macular degeneration, port-wine stains (PWS), and prostate cancer. Its efficacy is a complex function of photosensitizer (PS) uptake, oxygen concentration, PS-activating light dose and tissue optical properties. In order to non -invasively monitor PS distribution and photobleaching, blood vessel contraction and blood flow velocity for V-PDT response, a multimode optical imaging (MOI) system was developed to capture PS fluorescence image, narrow band image and laser speckle image, respectively. V-PDT in vivo studies were performed, which suggests that our MOI system is capable of monitoring dynamic response during V-PDT treatment.
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