A method for the determination of tiopronin content in drug by copper(І)-cuproine spectrophotometry is established. The hydrosulfuryl(-SH) in tiopronin can quantitatively can reduce Cu2+ to Cu+, Cu+ react with cuproine to form copper(І)- cuproine amaranth complex in the condition of pH=5.0, the content of tiopronin can be determinated by measuring the absorbance of copper(І)-cuproine amaranth complex. The optimum conditions for the determination of tiopronin by copper(І)-cuproine spectrophotometry are discussed. Under the optimal conditions, the content of tiopronin in drug is determined, and the results are satisfactory.
Cu2+ can be reduced to Cu+ by -SH (sulfhydryl) in cysteine, then neocuproine as the chromogenic reagent reacts with Cu+ to form the yellow complex. At the maximum absorption wavelength, the amount of the yellow complex is directly proportional to the added amount of cysteine in a certain range. According to this principle, the content of cysteine can be determined by spectrophotometry. Thus, a method for determination of cysteine by copper(I)–neocuproine spectrophotometry is established. Under the optimal conditions, this method is used to determinate the cysteine content in food, and the determination results are basically close to the result by pharmacopoeia method. The recovery rates of standard addition are 102.4%~104.3%.
An accurate, simple, fast spectrophotometric method for the determination of sulfite in food by silicon molybdenum heteropoly acid has been established. The various effect factors on the spectrophotometric determination of sulfite by silicon molybdenum heteropoly acid are investigated in detail. Under the optimum reaction conditions, silicon molybdenum heteropoly acid [H4Si(Mo3O10)4] formed by SiO32- and Mo7O246- is reduced by sulfite to form silicomolybdenum blue [H4Si(Mo3O10)2(Mo3O9)2]. The absorbance of silicomolybdenum blue is measured at 735.8 nm, and the content of sulfite can be determined based on the absorbance of silicomolybdenum blue. This proposed method has been applied to determinate of sulfite in food, and the results are satisfactory.
In acidic medium and under the catalysis of KBr, orange yellow 1 can be oxidized by KIO3 and this causes the solution to fade. A good linear relationship is obtained between the fading degree of the solution and KIO3 dosage. Hereby, a new method for the determination of KIO3 content in edible salt by fading spectrophotometry is established. The experimental results show that maximum absorption wavelength of orange yellow 1 is 490 nm in acidic medium, the linear relationship between the KIO3 mass concentration and the change value of absorbance is ΔΑ=-0.0402+0.0745C (μg/mL) when the mass concentration of KIO3 is 0.6000~2.000 μg/mL, and the linear correlation coefficient is 0.9987. This method had been successfully applied to determinate KIO3 in edible salt, and the results are satisfactory.
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