Dr. Naga S. Annamdevula Profile

Dr. Naga S. Annamdevula

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Publications (14)

Proceedings Article | 12 March 2024 Presentation + Paper

High-speed fluorescence excitation-scanning hyperspectral imaging microscopy using thin-film tunable filters

Santina Johnson, Naga Annamdevula, Thomas Rich, Chris Ballard, Silas Leavesley

Proceedings Volume 12846, 128460B (2024) https://doi.org/10.1117/12.3001600

KEYWORDS: Hyperspectral imaging, Tunable filters, Fluorescence, Microscopes, Optical filters, Light sources, Fluorescent markers, Imaging systems, Visualization, Light sources and illumination

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SPIE Journal Paper | 7 February 2023 Open Access

Multifaceted mirror array illuminator for fluorescence excitation-scanning spectral imaging microscopy

Marina Parker, Samuel A. Mayes, Craig Browning, Joshua Deal, Samantha Gunn-Mayes, Naga Annamdevula, Thomas Rich, Silas Leavesley

JBO, Vol. 28, Issue 02, 026502, (February 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.2.026502

KEYWORDS: Light emitting diodes, Microscopes, Fluorescence, Mirrors, Signal to noise ratio, Tunable filters, Imaging systems, Prototyping, Autofluorescence, Optical filters

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SignificanceHyperspectral imaging (HSI) technologies offer great potential in fluorescence microscopy for multiplexed imaging, autofluorescence removal, and analysis of autofluorescent molecules. However, there are also associated trade-offs when implementing HSI in fluorescence microscopy systems, such as decreased acquisition speed, resolution, or field-of-view due to the need to acquire spectral information in addition to spatial information. The vast majority of HSI fluorescence microscopy systems provide spectral discrimination by filtering or dispersing the fluorescence emission, which may result in loss of emitted fluorescence signal due to optical filters, dispersive optics, or supporting optics, such as slits and collimators. Technologies that scan the fluorescence excitation spectrum may offer an approach to mitigate some of these trade-offs by decreasing the complexity of the emission light path.AimWe describe the development of an optical technique for hyperspectral imaging fluorescence excitation-scanning (HIFEX) on a microscope system.ApproachThe approach is based on the design of an array of wavelength-dependent light emitting diodes (LEDs) and a unique beam combining system that uses a multifurcated mirror. The system was modeled and optimized using optical ray trace simulations, and a prototype was built and coupled to an inverted microscope platform. The prototype system was calibrated, and initial feasibility testing was performed by imaging multilabel slide preparations.ResultsWe present results from optical ray trace simulations, prototyping, calibration, and feasibility testing of the system. Results indicate that the system can discriminate between at least six fluorescent labels and autofluorescence and that the approach can provide decreased wavelength switching times, in comparison with mechanically tuned filters.ConclusionsWe anticipate that LED-based HIFEX microscopy may provide improved performance for time-dependent and photosensitive assays.

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Proceedings Article | 4 March 2022 Presentation + Paper

Measurement of agonist-induced Ca2+ signals in human airway smooth muscle cells using excitation scan-based hyperspectral imaging and image analysis approaches

Santina Johnson, D. Pleshinger, Josephine Jalkh, Zara Ijaz, Naga Annamdevula, Andrea Britain, C. Michael Francis, Deepak Deshpande, Silas Leavesley, Thomas Rich

Proceedings Volume 11964, 119640J (2022) https://doi.org/10.1117/12.2608276

KEYWORDS: Calcium, Hyperspectral imaging, Image analysis, Optical filters, Image filtering, Signal detection, Microscopy, Tunable filters, Spatial filters

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Proceedings Article | 4 March 2022 Paper

Improving visualization of cAMP gradients using algorithmic modelling

Patrick Howze, Naga Annamdevula, AnhVu Phan, D.J. Pleshinger, Thomas Rich, Silas Leavesley

Proceedings Volume 11964, 119640M (2022) https://doi.org/10.1117/12.2607772

KEYWORDS: Fluorescence resonance energy transfer, Data modeling, Visualization, Interference (communication), Finite element methods, Signal to noise ratio, Hyperspectral imaging, Lung, Signal detection, Sensors

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Proceedings Article | 4 March 2022 Presentation + Paper

Novel hyperspectral imaging approaches allow 3D measurement of cAMP signals in localized subcellular domains of human airway smooth muscle cells

Madison Howard, Naga Annamdevula, D. Pleshinger, Santina Johnson, Luke Beech, Raymond Penn, C. Michael Francis, Thomas Rich, Silas Leavesley

Proceedings Volume 11964, 119640I (2022) https://doi.org/10.1117/12.2608267

KEYWORDS: Fluorescence resonance energy transfer, Signal detection, Visualization, Imaging spectroscopy, Amplifiers, Hyperspectral imaging, Smoothing, Microscopes, Confocal microscopy, Venus

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Proceedings Article | 2 March 2022 Presentation + Paper

Hyperspectral imaging and adaptive thresholding to identify agonist-induced cAMP signals in pulmonary microvascular endothelial cells

N. Annamdevula, S. Johnson, D. Pleshinger, S. Castleberry, W. Russel, A. Britain, C. M. Francis, T. C. Rich, S. J. Leavesley

Proceedings Volume 11966, 1196604 (2022) https://doi.org/10.1117/12.2608292

KEYWORDS: Fluorescence resonance energy transfer, Hyperspectral imaging, Algorithm development, Signal to noise ratio, Image analysis, Vehicle control, Imaging spectroscopy, Visualization, Sensors, Computer programming

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Proceedings Article | 5 March 2021 Poster + Presentation + Paper

Effects of tissue autofluorescence on FRET efficiency estimates

D. J. Pleshinger, Naga Annamdevula, Silas Leavesley, Thomas Rich

Proceedings Volume 11649, 1164919 (2021) https://doi.org/10.1117/12.2583284

KEYWORDS: Fluorescence resonance energy transfer, Tissues, Biological research, Fluorescent proteins, Visible radiation, Venus, Resonance energy transfer, Molecular energy transfer, Kidney, Image analysis

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Proceedings Article | 5 March 2021 Presentation + Paper

Hyperspectral imaging approaches to characterize the distribution and expression of FRET in tissues isolated from a novel transgenic rat expressing a FRET-based probe

Naga Annamdevula, Aron Geurts, Silas Leavesley, Thomas Rich

Proceedings Volume 11649, 1164904 (2021) https://doi.org/10.1117/12.2583267

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Proceedings Article | 5 March 2021 Presentation + Paper

Assessing drug-cell and drug-tissue interactions through spectral FRET imaging

Silas Leavesley, Naga Annamdevula, Aron Geurts, D. J. Pleshinger, Thomas Rich

Proceedings Volume 11624, 116240D (2021) https://doi.org/10.1117/12.2583249

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Proceedings Article | 17 February 2020 Presentation + Paper

Comparison of spectral FRET microscopy approaches for single-cell analysis

Joshua Deal, Naga Annamdevula, Donald Pleshinger, John Robert Griswold, Aliyah Odom, Alia Tayara, Malvika Lall, Craig Browning, Marina Parker, Thomas Rich, Silas Leavesley

Proceedings Volume 11243, 112430Y (2020) https://doi.org/10.1117/12.2546308

KEYWORDS: Fluorescence resonance energy transfer, Confocal microscopy, Microscopy, Molecules, Luminescence, Molecular energy transfer, Data acquisition, Electronic filtering, Optical filters, Hyperspectral imaging

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Proceedings Article | 4 March 2019 Presentation + Paper

Hyperspectral imaging microscopy for measurement of localized second messenger signals in single cells

Thomas Rich, J. Griswold, Joshua Deal, Naga Annamdevula, Kathleen McAlister, Samuel Mayes, Craig Browning, Marina Parker, Silas Leavesley

Proceedings Volume 10881, 108811F (2019) https://doi.org/10.1117/12.2508052

KEYWORDS: Proteins, Signal to noise ratio, Signal detection, 3D metrology

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Proceedings Article | 17 February 2017 Paper

5D imaging approaches reveal the formation of distinct intracellular cAMP spatial gradients

Thomas Rich, Naga Annamdevula, Kenny Trinh, Andrea Britain, Samuel Mayes, John Griswold, Joshua Deal, Chase Hoffman, Savannah West, Silas Leavesley

Proceedings Volume 10070, 100700R (2017) https://doi.org/10.1117/12.2253164

KEYWORDS: Fluorescence resonance energy transfer, Hyperspectral imaging, Microscopy, Signal to noise ratio, 3D metrology, Visualization, Sensors, Venus, Fluorescent proteins, Imaging technologies, 3D image processing, Luminescence

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Proceedings Article | 16 February 2017 Presentation + Paper

Comparing methods for analysis of biomedical hyperspectral image data

Silas Leavesley, Brenner Sweat, Caitlyn Abbott, Peter Favreau, Naga Annamdevula, Thomas Rich

Proceedings Volume 10068, 100680S (2017) https://doi.org/10.1117/12.2252827

KEYWORDS: Hyperspectral imaging, Biomedical optics, Biological research, Spectroscopy, Microscopy, In vivo imaging, Basic research, Imaging systems, Sensors, Detection and tracking algorithms, Green fluorescent protein, Lung, Signal detection, Tissues, Image analysis

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Proceedings Article | 9 March 2016 Paper

Three-dimensional measurement of cAMP gradients using hyperspectral confocal microscopy

Thomas Rich, Naga Annamdevula, Andrea Britain, Samuel Mayes, Peter Favreau, Silas Leavesley

Proceedings Volume 9713, 97130O (2016) https://doi.org/10.1117/12.2213273

KEYWORDS: Confocal microscopy, 3D metrology, Microscopy, Image analysis, Microscopes, Hyperspectral imaging, 3D image processing, Stereoscopy, Visualization, Sensors, Signaling molecules, Fluorescence resonance energy transfer, Venus, Control systems

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Showing 5 of 14 publications

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