Here, we present a new handheld multiphoton endomicroscopic system designed for tumor diagnosis in the head and neck region. It consists of an approximate 25-cm-long rigid endomicroscopic probe with two variants (0° and 45° bended tip), connected to a handheld scan-head. The system can achieve a field of view ⪆600 µm for Coherent Anti-stokes Raman Scattering (CARS) and other nonlinear imaging modalities by a non-de-scanned detection and using a de-scanned confocal imaging channel to detect light from tissue labeled with Indocyanine Green (ICG). Furthermore, high-power femtosecond laser pulses can be transmitted through the system for precise tissue ablation which was considered in the optical design of the probe.
Here, we present a new handheld multiphoton endomicroscopic system for tumor diagnosis in the head and neck region. It consists of an approx. 25 cm long rigid endomicroscopic probe with two variants (0° and 45° bended tip), connected to a handheld scan-head. The system can achieve a field of view > 600 μm for coherent anti-Stokes Raman scattering (CARS) and other nonlinear imaging techniques by a non-descanned detection channel, and laser confocal imaging with indocyanine green (ICG) by a descanned detection channel. Furthermore, high-power femtosecond laser pulses can be transmitted through the system for precise tissue ablation without the risk of damaging the optical components.
Here, we report a new handheld endoscopic system for nonlinear multimodal imaging of the head and neck region. It has a long rigid endomicroscopic probe with two versions (0° and 45° bended tip), connecting with a compact scan-head of approx. 10×12×6 cm3 size. The rigid probe is 6 mm in diameter and 24 cm long and allows diffraction-limited multiphoton imaging of tissue with at least 430 μm field of view and sub-micron resolution. The signals of Coherent anti-Stokes Raman Scattering (CARS), second harmonic generation (SHG), and two-photon excited fluorescence (TPEF) are collected by a non-descanned detection path in the scan-head, and the fluorescence of Indocyanine green (ICG) labeled lesions is detected by a confocal descanned configuration. Furthermore, this system is capable of guiding high-power femtosecond laser pulses for tissue ablation without the risk of damaging optical glass components.
This work presents the design and implementation of an endoscopic probe for point-of-care diagnosis of bladder cancer, with an outer diameter of 4.5 mm that allows for in-vivo usage. This triple-modality device can deliver volumetric OCT images, optoacoustic tomograms, and single point Raman spectroscopy that target complementary biomarkers. The probe features a piezo-based fiber scanner, which delivers the illumination or excitation light for all modalities, with a maximum Field of View of 1.6 mm. The same path is used for light collection for OCT imaging. A separate fiber is used for detection of the Raman signals, while two additional fibers with microcavity tips sense the ultrasonic waves for optoacoustic tomography. A hyperchromatic micro-optical objective provides a working distance optimized for each modality. The probe housing is produced by selective laser-induced etching of fused silica.
A miniature endomicroscope is presented that combines a large field-of-view (up to 1.7 mm) for OCT-imaging and a high-resolution mode with 360 μm field-of-view (NA = 0.5) for multi-photon fluorescence or OCT imaging. The 4.7x magnification variation is achieved by the axial positioning of an inner micro-optical lens group using an integrated electro-magnetic z-actuator. A reverse fiber-optic piezotube-scanner with minimized length is employed for the image acquisition by resonant spiral scanning. With the probe diameter of 2.7 mm and a rigid length of about 60 mm, the approach may pave the way to clinical applications of these two modalities in a single probe.
We demonstrate a flexible stand-alone, minimally invasive video-endomicroscope with an outer diameter of
1.6 mm and a length of the rigid tip of 6.7 mm that enables surgeons and biologists to image hardly accessible
regions in-vivo in epifluorescence mode. The 60 mg light device improves state-of-the-art objectives by a
double deflection approach using a side-fire fiber in combination with spherical microlenses, GRIN-lenses
with a specific adapted gradient index profile and an extremely miniaturized chip-on-the-tip camera to
achieve an excellent imaging quality. A high NA of 0.7 enables the observation of subcellular features within
the entire field of view with a diameter of 183 μm, assure a bright and high-contrast image and promise a
good overview during the intervention. Ex-vivo measurements of biological samples confirmed the
functionality of the probe.
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